吉林大学学报(工学版) ›› 2010, Vol. 40 ›› Issue (03): 868-0872.

• paper • Previous Articles     Next Articles

Screening of xylitolproducing strain and optimization of its fermentation conditions

ZHAO Shou-jing, HOU Kun, LIANG Yan-long| XU Li-xin| QIAN Yan-chun   

  1. College of Biological and Agricultural Engineering, Jilin University,Changchun 130022, China
  • Received:2009-01-13 Revised:2009-04-13 Online:2010-05-01 Published:2010-05-01

Abstract:

A yeast, which can perform biotransformation from xylose to xylitol, was isolated from corncob and was identified as trichosporon coremiiforme by morphological identification and 26S rDNA D1/D2 domain sequence analysis. Besides, the optimal fermentation conditions were determined through single factor test and orthogonal experiment. The optimal conditions are as follows, carbon source concentration: 40g/l, nitrogen source concentration: 6g/l, initial pH: 7.0 and inoculum size: 6%.

Key words: bioengineering, xylitol, biotransformation, trichosporon coremiiforme

CLC Number: 

  • Q815
[1] LU Chao, ZHAO Shou-jing, ZHANG Xiao-jia, DU Peng-chuan, WANG Xue-song. Construction of engineering bacteria containing Panax ginseng 3-O-UGT1 gene RNA interference plant expression vector [J]. 吉林大学学报(工学版), 2018, 48(1): 344-348.
[2] WANG Shu-jie, ZHANG Wei, HE Hai-bing, WANG Wan-shu, ZHANG Miao, HU Xin-yu. Effection of typical plants leaves on pesticide wetting speciality and retentivity [J]. 吉林大学学报(工学版), 2013, 43(增刊1): 564-568.
[3] ZHAO Shou-jing, CAO Hao-jie, HE Mu-yang, WANG Le. Engineering bacteria construction of ginseng β-AS gene RNA interference plant expression vector [J]. , 2012, (06): 1608-1612.
[4] ZHANG Qiang|Anders Thygesen|Anne Belinda Thomsen. Enzymatic hydrolysis and ethanol fermentation from corn stover pretreated by wet oxidation [J]. 吉林大学学报(工学版), 2011, 41(4): 1189-1192.
[5] LIANG Yan-long, ZHAO Shou-jing, XU Li-xin, LIU Li, HE Mu-yang, SUN Yao. Construction of engineered yeast producing ginsenoside precursor [J]. 吉林大学学报(工学版), 2011, 41(05): 1513-1516.
[6] ZHAO Shou-jing,HOU Chun-xi,XU Li-xin,LIANG Yan-long,QIAN Yan-chun,SUN Yao. Effects of suppressing oleanane-type ginsenoside biosynthesis on dammarane-type ginsenoside production [J]. 吉林大学学报(工学版), 2011, 41(03): 865-868.
[7] XU Li-Xin, ZHAO Shou-Jing, LIANG Yan-Long, QIAN Yan-Chun, JIN Shi-Kun. Effect of extrinsic regulators on growth and ginsenoside content of hairy roots of Panax ginseng [J]. 吉林大学学报(工学版), 2010, 40(06): 1619-1623.
[8] Yan Hong-sen,Zhang Shou-qin,Hou Li-li,Luo Xiao-pei1,Liang Qing . Effect of extraction technology on the stability and antioxidant
activity of extract of Hedyotis diffusa willd
[J]. 吉林大学学报(工学版), 2008, 38(增刊): 214-0218.
[9] Sun Li,Zhao Lu. Effect of medium on callus of two different plants with orthogonal test design combinations [J]. 吉林大学学报(工学版), 2006, 36(04): 628-634.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!