吉林大学学报(工学版) ›› 2018, Vol. 48 ›› Issue (1): 344-348.doi: 10.13229/j.cnki.jdxbgxb20161203

• Orginal Article • Previous Articles    

Construction of engineering bacteria containing Panax ginseng 3-O-UGT1 gene RNA interference plant expression vector

LU Chao1, ZHAO Shou-jing1, 2, ZHANG Xiao-jia2, DU Peng-chuan2, WANG Xue-song1, 2   

  1. 1.College of Biological and Agricultural Engineering, Jilin University, Changchun 130022,China;
    2.College of Life Sciences, Jilin University, Changchun 130012,China
  • Received:2016-11-05 Online:2018-02-26 Published:2018-02-26

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Abstract: The ginseng 3-O-UGT1 gene (pg3-O-UGT1) is a key enzyme for controlling the synthesis of protopanaxadiol-group in Panax ginseng. In general, the protopanaxadiol-group ginsenosides have pharmaceutical activities that are opposite those of the protopanaxatriol-group. In this study, the ginseng 3-O-UGT1 gene RNA interference plant expression vector was constructed using Reverse Transcription Polymerase Chain Reaction (TR-PCR) and restriction enzyme digestion connection techniques. Then, a 3-O-UGT1 gene RNAi plant expression vector engineered bacteria was harvested after being transformed into the recombinant expression vectors. This work provides the foundation for the induction of ginseng explants and the mechanism of ginsenosides synthesis.

Key words: bioengineering, ginsenosides, RNAi plant expression vector, glycosyltransferase, engineering bacteria

CLC Number: 

  • Q81
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