Abstract:

In order to improve the activity, stability and reusability of watersoluble βglucosidase in the reaction of alkylpolyglycoside synthesis, chitosan and sodium alginate, a new composite vector (alginatechitosanalginate, referred to as ACA),was used  to immobilize βglucosidase, and the mathematical optimization model of immobilized enzyme's technological parameters and mathematical model of enzyme activity (glucose conversion rate) were studied in detail. The results show that chitosan, glutaraldehyde, trigger time in CaCl2 and sodium alginate are influence factors of glucose conversion rate successively; optimization parameters of immobilized enzyme are chitosan of 0.2 g, sodium alginate of 0.6 g, trigger time in CaCl2 of 30 min, glutaraldehyde of 0.25 g/L, and glucose conversion rate is 85.8%; chitosan and wall materials solution show a positive interaction among trigger time in CaCl2, glutaraldehyde and sodium alginate; wall materials solution show a negative interaction between trigger time in CaCl2 and glutaraldehyde; optimized glucose conversion rate and main factor code value are regression mathematical model of variables secondary universal rotary combination design and regression mathematical model of variables and test effect.

Key words: food industry, food emulsifying agent alkyl polyglycoside, water soluble β-glucosidase, ACA microcapsule immobilization technics, glucose conversion rate