亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数

亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数

周新¹,汪子明¹,邹明强²,郑静¹,宋大千¹,郑健¹,王璐¹,张寒琦¹

(1. 吉林大学　(1. 吉林大学　化学学院分析化学系，长春 130012； 2. 中国检验检疫科学研究院检测新技术与装备研究所，北京 100025)

收稿日期:2005-01-21 修回日期:1900-01-01 出版日期:2005-09-26 发布日期:2005-09-26
通讯作者: 张寒琦

Determination of Binding Constant between Human Serum Albumin and Flavonoid Compounds by Affinity Capillary Electrophoresis

ZHOU Xin¹,WANG Zi-ming¹,ZOU Ming-qiang²,ZHENG Jing¹,SONG Da-qian¹,ZHENG Jian¹,WANG Lu¹,ZHANG Han-qi¹

(1． Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China;　2． Institute of Inspection Technology and Equipment, Chinese Academy of Inspection and Quarantine, Beijing 100025, China)

Received:2005-01-21 Revised:1900-01-01 Online:2005-09-26 Published:2005-09-26
Contact: ZHANG Han-qi

摘要/Abstract

摘要： 采用亲和毛细管电泳技术，对木犀草素、槲皮素和山柰酚3种黄酮类化合物与人血清白蛋白（HSA）的结合常数进行研究，利用药物淌度变化和蛋白浓度的关系，计算得到木犀草素、槲皮素和山柰酚与HSA的结合常数分别为9.76×10⁵mol/L，1.24×10⁶mol/L和3.79×10⁵mol/L. 结果表明，此法可以作为研究药物与蛋白相互作用的一种简便可行方法.

关键词: 亲和毛细管电泳, 木犀草素, 槲皮素, 山柰酚, 人血清白蛋白, 结合常数

Abstract: The binding constants between human serum albumin (HSA) and each of three flavonoid compounds were determined by affinity capillary electrophoresis (ACE). The binding constants are 9.76×10⁵mol／L, 1.24×10⁶mol／L and 3.79×10⁵ mol／L for luteolin, quercetin and kaempferol, respectively. The results show that ACE is a useful method to study the interaction between drug and protein.

Key words: affinity capillary electrophoresis, luteolin, quercetin, kaempferol, human serum albumin, binding constant

中图分类号:

O657.8

周新,汪子明,邹明强,郑静,宋大千,郑健,王璐,张寒琦. 亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数[J]. J4, 2005, 43(05): 669-672.

ZHOU Xin,WANG Zi-ming,ZOU Ming-qiang,ZHENG Jing,SONG Da-qian,ZHENG Jian,WANG Lu,ZHANG Han-qi. Determination of Binding Constant between Human Serum Albumin and Flavonoid Compounds by Affinity Capillary Electrophoresis[J]. J4, 2005, 43(05): 669-672.

[1]	汤峰, 吴静义, 张剑, 张宏桂, 岳永德. 水竹叶的化学成分[J]. 吉林大学学报(理学版), 2013, 51(04): 724-726.
[2]	周秀清, 程建华, 孙磊, 徐昊妍, 金海燕. 用光谱法研究异鼠李素与牛血清白蛋白的相互作用及几种金属离子对反应的影响[J]. J4, 2008, 46(05): 983-987.
[3]	郭晔, 陈艳华, 田媛, 高德江, 邓新煜, 张寒琦, 于爱民，盛韧. 以聚二烯丙基二甲基氯化铵为探针测定人血清中总蛋白的含量[J]. J4, 2007, 45(04): 673-675.
[4]	毕淑云,，宋大千，魏士刚，王桂芬，陈铁，胡秀丽,，刘忠英，乔春玉，张寒琦. 用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应[J]. J4, 2006, 44(03): 493-496.
[5]	周新，丁兰，汪子明，白利飞，郑健，田媛，郑静，张寒琦. 高良姜中槲皮素的超声雾化萃取[J]. J4, 2005, 43(02): 228-231.