DhHP-6及Exenatide对胰岛β细胞的协同作用

吉林大学学报(理学版)

DhHP-6及Exenatide对胰岛β细胞的协同作用

雷莉妍^1, 王迪^1, 张广吉^1, 郭佑铭¹, 王丽萍^1,2

1. 吉林大学生命科学学院, 长春 130012； 2. 吉林大学中日联谊医院急救医学科, 长春 130033

收稿日期:2013-09-29 出版日期:2014-07-26 发布日期:2014-09-26
通讯作者: 王丽萍 E-mail:wanglp@jlu.edu.cn

Synergistic Effects of DhHP6 and Exenatideon Pancreatic β Cells

LEI Liyan¹, WANG Di¹, ZHANG Guangji¹, GUO Youming ¹, WANG Liping^1,2

1. College of Life Science, Jilin University, Changchun 130012, China；2. Department of Emergency, ChinaJapan Union Hospital, Jilin University, Changchun 130033, China

Received:2013-09-29 Online:2014-07-26 Published:2014-09-26
Contact: WANG Liping E-mail:wanglp@jlu.edu.cn

摘要/Abstract

摘要：

采用葡萄糖和棕榈酸钠（Glu-Pal）联合诱导大鼠胰岛瘤RINm-5F细胞凋亡, 用噻唑蓝比色(MTT)实验检测次血红素六肽（DhHP-6）和Exenatide分别或联合作用对细胞凋亡的影响. 结果表明: DhHP-6和Exenatide均显著抑制Glu-Pal诱导β细胞凋亡, 但二者无协同作用; DhHP-6和Exenatide均明显促进RINm-5F细胞增殖, 且有显著的协同作用.

关键词: 次血红素六肽, Exenatide, 胰岛&beta, 细胞, 协同作用

Abstract:

Apoptosis of pancreatic β cell RINm-5F was induced by glucose coupled with sodium palmitate. Effects of DhHP-6 or/and Exenatide on the apoptosis of RINm-5F cell were detected by methyl thiazolyl tetrazolium (MTT) assay. DhHP6 and Exenatide decreased the apoptosis induced by Glu-Pal significantly without no synergistic effect between them. In addition, we also found that DhHP6 and Exenatide both stimulated the proliferation of RINm-5F cells, and there was a synergistic effect between them.

Key words: DhHP-6, Exenatide, pancreatic β cell, synergistic effect

中图分类号:

雷莉妍, 王迪, 张广吉, 郭佑铭, 王丽萍. DhHP-6及Exenatide对胰岛β细胞的协同作用[J]. 吉林大学学报(理学版), 2014, 52(04): 835-839.

LEI Liyan, WANG Di, ZHANG Guangji, GUO Youming,WANG Liping. Synergistic Effects of DhHP6 and Exenatideon Pancreatic β Cells[J]. Journal of Jilin University Science Edition, 2014, 52(04): 835-839.

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