重组TgoDNA聚合酶的特性

重组TgoDNA聚合酶的特性

盛艳敏¹,⁵，许月²，廖俊杰³，吴英杰⁴,　李彦舫⁵

1. 长春师范学院生物系，长春 130032； 2. 吉林大学生命科学学院，长春　130021； 3. 广州轻工职业技术学院食品与生物工程系，广州 510300； 4. 西奈山医学院医学系，纽约 10029； 5. 吉林大学植物科学学院, 长春 130062

收稿日期:2006-03-17 修回日期:1900-01-01 出版日期:2006-11-26 发布日期:2006-11-26
通讯作者: 李彦舫

PCR Characters of a Recombined Tgo DNA Polymerase

SHENG Yanmin¹,⁵, XU Yue², LIAO Junjie³, WU Yingjie⁴, LI Yanfang⁵

1.　Department of Biology, Changchun Normal College, Changchun 130032, China;2. College of Life Science, Jilin University, Changchun 13002１, China; 3. Department of Foodstuff and Biotechnology， Guangzhou Light Industry Employee Technique College, Guangzhou 510300, China; 4. Department of Medicine, Mount Sinai School of Medicine, New York 10029, USA； 5. College of Plant Science, Jilin University, Changchun 1300６2, China

Received:2006-03-17 Revised:1900-01-01 Online:2006-11-26 Published:2006-11-26
Contact: LI Yanfang

摘要/Abstract

摘要： 检测了重组Tgo酶的扩增性能、耐热性以及长距离PCR能力，结果表明, 该重组酶扩增性能与Taq酶相当；其耐热性极强，在 95 ℃ 40 min，仍具有很强的聚合活性；利用重组酶在常规PCR条件下，成功扩增了5.3　kb的拟南芥Timeless基因片段.

关键词: 重组TgoDNA聚合酶, 扩增性能, 耐热性, PCR

Abstract: The amplification capability, the heat tolerance and the ability to long PCR of Tgo DNA polymerase were examined. The results reveal that Tgo DNA polym erase has the same amplification function asTaq enzyme but with a high fidelity. It also has a strong resistance to heat. Its amplification function would not be notably affected at 95 ℃ for 40 min. Via the recombinant enzyme, the 5.3　kb segment of Timeless gene was successfully amplified from Arabidopsis under the general conditions of PCR.

Key words: recombinant Tgo DNA polymerase, amplification capability, heat tolerance, P CR

中图分类号:

盛艳敏,，许月，廖俊杰，吴英杰,　李彦舫. 重组TgoDNA聚合酶的特性[J]. J4, 2006, 44(06): 1008-1010.

SHENG Yanmin,, XU Yue, LIAO Junjie, WU Yingjie, LI Yanfang. PCR Characters of a Recombined Tgo DNA Polymerase[J]. J4, 2006, 44(06): 1008-1010.

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