新型DNA聚合酶Tgo的扩增忠实性测定

新型DNA聚合酶Tgo的扩增忠实性测定

盛艳敏¹，许月²，廖俊杰³，吴英杰⁴, 李彦舫⁵

1.长春师范学院生物系，长春　130032； 2. 吉林大学生命科学学院，长春　130023； 3.　广州轻工职业技术学院食品与生物工程系，广州　510300； 4.　宾夕法尼亚大学生物系, 费城 PA19104； 5.　吉林大学农学部植物基因工程研究中心, 长春 130062

收稿日期:2004-03-15 修回日期:1900-01-01 出版日期:2005-01-26 发布日期:2005-01-20
通讯作者: 李彦舫

Polymerase Chain Reaction Fidelity of Novel Tgo DNA Polymerase

SHENG Yan-min^1,5, XU Yue², LIAO Jun-jie^{3^{, WU Ying-jie⁴, LI Yan-fang⁵}}

1. Department of Biology, Changchun Normal College, Changchun 130032, China;2. College of Life Science, Jilin University, Changchun 130023, China; 3. Department of Foodstuff and Biotechnology, Guangzhou Light Industry Employee Technique College, Guangzhou 510300, China; 4. Department of Biology, University of Pennsylvania, Philadelphia PA19104, USA； 5. Research Center of Plant Genetic Engineering, Department of Agriculture, Jilin University, Changchun 130062, China

Received:2004-03-15 Revised:1900-01-01 Online:2005-01-26 Published:2005-01-20
Contact: LI Yan-fang

摘要/Abstract

摘要： 用分子克隆技术从Thermococcus gorgonarius克隆并表达重组Tgo DNA聚合酶，得到纯化的热稳定Tgo DNA聚合酶. 利用重组酶成功扩增了质粒pUC19、野大麦Na⁺/H⁺逆向运转蛋白基因、小鼠Cx37基因和人CYT2C9基因. 该酶的扩增性能与Taq酶相当，而扩增忠实性分别比Taq酶、 Pfu酶高30倍和1.6倍.

关键词: TgoDNA聚合酶, 扩增性能, 扩增忠实性

Abstract: Thermostable Tgo DNA polymerase is obtained from Thermococcus gorgonarius by the molecular cloning and express technology. Plasmid19, wild barley Na+/H⁺ reverse transporter gene, mous Cx37 gene and human CYT2C9 gene were successfully amplified with recombinase. The amplification capability and amplification fidelity of the enzyme were compared with those of Taq enzyme. The result indicates that the amplification capability of Tgo enzyme is the same as that of Taq enzyme. But the amplification fidelity of Tgo enzyme is over 30 times higher than that of Taqenzyme and 1.6 times higher than that of Pfu.

Key words: Tgo DNA polymerase, amplification capability, amplification fidelity

中图分类号:

盛艳敏，许月，廖俊杰，吴英杰, 李彦舫. 新型DNA聚合酶Tgo的扩增忠实性测定[J]. J4, 2005, 43(01): 101-105.

SHENG Yan-min, XU Yue, LIAO Jun-jie, WU Ying-jie, LI Yan-fang. Polymerase Chain Reaction Fidelity of Novel Tgo DNA Polymerase[J]. J4, 2005, 43(01): 101-105.