液相色谱-串联质谱法测定大鼠血浆中莪术醇的浓度

液相色谱-串联质谱法测定大鼠血浆中莪术醇的浓度

王岩¹，李馨², 刘银燕³, 胡立刚⁴, 滕国生⁴, 王江⁴

1. 吉林大学第一医院药剂科, 长春 130021； 2. 吉林大学第二医院药剂科, 长春 130041； 3. 吉林大学药学院, 长春 130021; 4. 吉林大学生命科学院, 长春 130021

收稿日期:2006-08-03 修回日期:1900-01-01 出版日期:2007-05-26 发布日期:2007-05-26
通讯作者: 王岩

Determination of Ｃurcumol Ｃoncentration in Ｒat Ｐlasma by Ｌiquid Ｃhromatographytandem Ｍass Ｓpectrometry

WANG Yan¹, LI Xin², LIU Yinyan³, HU Ligang⁴, TENG Guosheng⁴, WANG Jiang⁴

1． Agentia Section, The First Hospital, Jilin University, Changchun 130021, China; 2． Agentia Section, The Second Hospital, Jilin University, Changchun 130041, China; 3． College of Pharmaceutical, Jilin University, Changchun 130021, China; 4． College of Life Science, Jilin University, Changchun 130021, China

Received:2006-08-03 Revised:1900-01-01 Online:2007-05-26 Published:2007-05-26
Contact: WANG Yan

摘要/Abstract

摘要： 建立了一个快速、准确、灵敏的液相色谱串联质谱测定鼠血浆中莪术醇的分析方法. 血浆样品经乙腈沉淀蛋白后，以甲醇-乙腈-10 mmol/L乙酸铵溶液（体积比： 42.5 ∶42.5 ∶15）为流动相，采用反相色谱柱Nucleosil C¹⁸分离，通过电喷雾离子化源的四极杆串联质谱仪，以多反应监测方式检测. 线性范围为2.5~500.0 μg／L，日间和日内精密度< 4.79%，平均提取回收率为100.7%.

关键词: 莪术醇, 液相色谱串联质谱, 大鼠血浆

Abstract: A rapid, simple and sensitive liquid chromatography/tandem mass spectro metry　(LC/MS/MS) method was developed for the determination of curcumol in rat plasma. Samples of the plasma were deproteinated with acetonitrile, the analytes were then analyzed by reversedphase HPLC on a Hypersil ODS column with methanol-ace tonitrile-10 mmol/L ammonium acetate　（volume ratio: 42.5 ∶42.5 ∶15）　as mobile phase. Detection was carried out by multiple reaction monitoring on a API4000 LC/MS/MS system with an electrospray ionization (ESI) interface. The assay was linear in a range of 2.5～500.0 μg／L, intra and interday precisions over this range were < 4.79%, the average recovery was 100.7%.

Key words: curcumol, liquid chromatographytandem mass spectrometry, rat plasma

中图分类号:

R917.101

王岩，李馨, 刘银燕, 胡立刚, 滕国生, 王江. 液相色谱-串联质谱法测定大鼠血浆中莪术醇的浓度[J]. J4, 2007, 45(03): 497-500.

WANG Yan, LI Xin, LIU Yinyan, HU Ligang, TENG Guosheng, WANG Jiang. Determination of Ｃurcumol Ｃoncentration in Ｒat Ｐlasma by Ｌiquid Ｃhromatographytandem Ｍass Ｓpectrometry[J]. J4, 2007, 45(03): 497-500.