RP-HPLC法测定不同生长期尾叶香茶菜中二萜类成分的含量

RP-HPLC法测定不同生长期尾叶香茶菜中二萜类成分的含量

桂明玉，李绪文，王宝珍，徐吉庆，金永日

吉林大学化学学院，长春 130021

收稿日期:2006-03-09 修回日期:1900-01-01 出版日期:2007-01-26 发布日期:2007-01-26
通讯作者: 桂明玉

Content of Diterpenoids in Rabdosia Excisa in Different Growth Periods via RP-HPLC

GUI Mingyu， LI Xuwen， WAＮＧ Baozhen， XU Jiqing， JIN Yongri

College of Chemistry, Jilin University, Changchun 130021, China

Received:2006-03-09 Revised:1900-01-01 Online:2007-01-26 Published:2007-01-26
Contact: GUI Mingyu

摘要/Abstract

摘要： 采用反相高效液相色谱法测定了不同生长期尾叶香茶菜叶的4种主要二萜类成分kamebakaurin(Ⅰ), excisanin A(Ⅱ)， rabdokunmin C(Ⅲ)和kamebanin(Ⅳ)的含量. 结果表明，不同生长期尾叶香茶菜叶中二萜类成分含量发生变化. 化合物Ⅰ和Ⅱ在7月上旬含量最高；化合物Ⅲ在6月初到8月中旬含量变化不大，但8月上旬含量开始下降；化合物Ⅳ在6月中旬含量最高， 7月上旬含量开始下降. 7月上旬尾叶香茶菜中总二萜类成分含量最高.

关键词: 尾叶香茶菜, 二萜, 反相高效液相色谱, 含量变化

Abstract: Four diterpenoids kamebakaurin (Ⅰ), excisanin A (Ⅱ)， rabdokunmin C (Ⅲ) and kamebanin (Ⅳ) in Rabdosia excisa in different growth periods were determined via RPHPLC. The separation was performed on a ZORBAX Extend C18 column (250 mm × 4.6 mm， 5 μm), with mixture 23% acetonitrile and 77% water as mobile phase at a rate of 1.2 mL／min and 25 ℃， the wavelength for the measurement w as 230 nm.　The results show that the content of the four diterpenoids in Rabdosia e xcisa are different in different growth periods. Kamebakaurin (Ⅰ) and excisanin A (Ⅱ) could reach the highest content respectively in the first ten days of July; Rabdokunmin C (Ⅲ) has a little variation from the begin of June to the beg in of August, then, the content would decrease; Kamebanin (Ⅳ) could reach the highest content in the middle ten days of June, the content would decrease then. The total content of the four diterpenoids is the highest in the first ten days of July.

Key words: Rabdosia excisa (Maxim.) Hara, diterpenoids, RP-HPLC, variation

中图分类号:

R917

桂明玉，李绪文，王宝珍，徐吉庆，金永日. RP-HPLC法测定不同生长期尾叶香茶菜中二萜类成分的含量[J]. J4, 2007, 45(01): 125-127.

GUI Mingyu， LI Xuwen， WAＮＧ Baozhen， XU Jiqing， JIN Yongri. Content of Diterpenoids in Rabdosia Excisa in Different Growth Periods via RP-HPLC[J]. J4, 2007, 45(01): 125-127.