CW2M3分泌新型淀粉酶的最适条件及酶的应用条件

CW₂M₃分泌新型淀粉酶的最适条件及酶的应用条件

滕利荣¹，高朝辉¹，²，王阳¹，²，孟庆繁¹，白挨玺¹，张应玖¹，²

1.　吉林大学生命科学学院，长春 130012； 2．　吉林大学分子酶学工程教育部重点实验室，长春　130021

收稿日期:2005-12-26 修回日期:1900-01-01 出版日期:2006-09-26 发布日期:2006-09-26
通讯作者: 张应玖

Opimization Conditions to Secrete and Utilize Neoamylase from CW₂M₃

TENG Lirong¹, GAO Chaohui¹,²， WANG Yang¹,²， MENG Qingfan¹，　 BAI Aixi¹，　ZHANG Yingjiu¹,²

1. College of Life Science， Jilin University， Changchun 130012, China; 2. Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University， Changchun 130021, China

Received:2005-12-26 Revised:1900-01-01 Online:2006-09-26 Published:2006-09-26
Contact: ZHANG Yingjiu

摘要/Abstract

摘要： 从原筛选的产酶菌株CW₂出发，经多次紫外诱变、初筛和复筛后，获得了变异菌株CW₂M₃，其产酶水平为原菌株的332.7％， 且具有良好的遗传稳定性. 薄层层析证明，CW₂M₃分泌的新型淀粉酶能有效地催化淀粉降解产生异麦芽低聚糖，产物主要包括异麦芽糖、潘糖、异麦芽三糖等. 用正交法结合薄层层析法确定了各因素对CW₂M₃产酶水平的影响，结果表明，培养温度是显著因素，CW₂M₃的最适产酶条件为：用牛肉膏培养基（牛肉膏0.7%、蛋白胨0.7%、 NaCl 0.5%、 pH 7.0）培养，温度40 ℃，时间12　h. 用该酶催化淀粉转化为异麦芽低聚糖时，酶促反应时间和温度均是显著影响因素，酶催化淀粉降解的最适条件为: 温度65 ℃，酶促反应1.0　h， 体系pH为7.0.

关键词: 新型淀粉酶, 异麦芽低聚糖, bacillusthermolicheniformis

Abstract: Strain CW₂M₃ was mutagenised from strain bacillus thermolicheniformis CW₂ by ultraviolet ray, and the activity of secreted neoamylase by CW₂M₃ is increased by 232.7% compared with that of CW₂ under the identical conditions. Moreover, strain CW₂M₃ is genetically stabile. The results of thin layer chromatography of the products from starch catalyzed by the neoamylase demonstrate that the neoamylase can be used to catalyze starch to produce isomaltooligosaccharide, including isomaltose, panose, isomaltotriose, from starch. The optimum conditions under which the neoamylase was secreted by CW₂M₃ were confirmed by orthogonal tests. The results show that the most important parameter to secrete the neoamylase is temperature for culturingCW₂M₃ and the optimum conditions to secrete the neoamylase are as follows. The strain CW₂M₃ should be cultured in the medium of 0.7% brewis, 0.7% peptone, 0.5% NaCl, pH 7.0 at 40 ℃ for 12 h, whereas the most important parameter to utilize the neoamylase is catalytic duration and the optimum conditions to utilize the neoamylase are in pH 7.0 starch system, at 65 ℃ for 1.0 h. 

Key words: neoamylase, isomaltooligosaccharide,  bacillus thermolicheniformis

中图分类号:

滕利荣，高朝辉，，王阳，，孟庆繁，白挨玺，张应玖，. CW₂M₃分泌新型淀粉酶的最适条件及酶的应用条件[J]. J4, 2006, 44(05): 834-838.

TENG Lirong, GAO Chaohui,， WANG Yang,， MENG Qingfan，　 BAI Aixi，　ZHANG Yingjiu,. Opimization Conditions to Secrete and Utilize Neoamylase from CW₂M₃[J]. J4, 2006, 44(05): 834-838.

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CW₂M₃分泌新型淀粉酶的最适条件及酶的应用条件

Opimization Conditions to Secrete and Utilize Neoamylase from CW₂M₃

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