6A,6A′-苯胺-6B,6B′-硒桥联-β-CD底物的特异性

吉林大学学报(理学版)

6A,6A′-苯胺-6B,6B′-硒桥联-β-CD底物的特异性

吕绍武¹, 吕冰聪^1, 宗慧¹, 陶进², 王宏龄²

1. 吉林大学分子酶学工程教育部重点实验室, 长春 130012; 2. 玉米深加工国家工程研究中心, 长春 130033

收稿日期:2013-03-11 出版日期:2013-09-26 发布日期:2013-09-17
通讯作者: 吕绍武 E-mail:lvsw@jlu.edu.cn

Substrate Specificity of 6A,6A′-Dianilino-6B,6B′-diselenide-bis-β-cyclodextrin

LV Shaowu¹, LV Bingcong^1, ZONG Hui ¹, TAO Jin², WANG Hongling²

1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education,Jilin University, Changchun 130012, China;2. National Engineering Research Center of Maize Further Processing, Changchun 130033, China

Received:2013-03-11 Online:2013-09-26 Published:2013-09-17
Contact: LV Shaowu E-mail:lvsw@jlu.edu.cn

摘要/Abstract

摘要：

运用双酶偶联体系分别测定6A,6A′-二苯胺-6B,6B′-二硒桥联-β-CD（6-AnSeCD）催化谷胱甘肽（GSH）还原过氧化氢（H₂O₂）、叔丁基过氧化氢（t-BuOOH）和枯烯过氧化氢（CumOOH）3种结构不同氢过氧化物的谷胱甘肽过氧化物酶（GPx）活力, 并考察6-AnSeCD催化这3种氢过氧化物的稳态动力学. 结果表明: 6AnSeCD还原CumOOH的GPx活力最高; 6-AnSeCD的催化机制为乒乓机制; 6AnSeCD的假一级反应速度常数（k_max）、米氏常数（K_m）和二级反应速率常数（k）均显示CumOOH为6-AnSeCD的最适底物.

关键词: 谷胱甘肽过氧化物酶, 模拟物, 环糊精, 稳态动力学

Abstract:

The gluathione peroxidase (GPx) activities of 6A,6A′-dianilino-6B,6B′-diselenide-bis-β-cyclodextrin (6-AnSeCD) in the reduction of H₂O₂, tertbutylhydroperoxide (t-BuOOH) and cumenyl hydroperoxide (CumOOH) by glutathione (GSH) were assessed in classical coupled reductase assay, and the steadystate kinetics of 6AnSeCD in the reduction of three hydrogen peroxides was studied. The 6-AnSeCD displayed best GPx activity for the reduction of CumOOH by GSH. A pingpong mechanism was observed in the steadystate kinetic studies on the 6-AnSeCDcatalyzed reactions. We further compared k_max, K_m and secondorder rate constant of 6-AnSeCD. The results show the CumOOH is the most preferred substrate for 6-AnSeCD.

Key words: gluathione peroxidase, mimic, cyclodextrin, steady state kinetics

中图分类号:

吕绍武, 吕冰聪, 宗慧, 陶进, 王宏龄. 6A,6A′-苯胺-6B,6B′-硒桥联-β-CD底物的特异性[J]. 吉林大学学报(理学版), 2013, 51(05): 961-964.

LV Shaowu, LV Bingcong, ZONG Hui, TAO Jin, WANG Hongling. Substrate Specificity of 6A,6A′-Dianilino-6B,6B′-diselenide-bis-β-cyclodextrin[J]. Journal of Jilin University Science Edition, 2013, 51(05): 961-964.

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