吉林大学学报(理学版)

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L-丝氨酸与L-天门冬氨酸对羟基磷灰石仿生矿化的影响

吴晓光1, 李毅1, 陶红2, 赵旭2, 闫秀娟1, 王珂1   

  1. 1. 吉林大学 口腔医院口腔科, 长春 130021; 2. 吉林大学 化学学院, 长春 130012
  • 收稿日期:2013-11-04 出版日期:2014-03-26 发布日期:2014-03-20
  • 通讯作者: 赵旭 E-mail:zhaoxu@jlu.edu.cn

Effects of L-Ser and L-Asp on the Crystallization of Hydroxyapatite

WU Xiaoguang1, LI Yi1, TAO Hong2, ZHAO Xu2, YAN Xiujuan1, WANG Ke1   

  1. 1. Department of Pediatric Dentistry, The Affiliated Hospital of Stomatology, Jilin University,Changchun 130021, China; 2. College of Chemistry, Jilin University, Changchun 130012, China
  • Received:2013-11-04 Online:2014-03-26 Published:2014-03-20
  • Contact: ZHAO Xu E-mail:zhaoxu@jlu.edu.cn

摘要:

根据仿生合成原理, 以L-丝氨酸(L-Ser)和L-天门冬氨酸(L-Asp)为有机基质, 通过气相扩散法制备碳羟基磷灰石晶体, 并在牙釉质片上进行仿生再矿化. 通过场发射扫描电子显微镜(FESEM)、 X射线衍射(XRD)和Fourier变换红外光谱(FTIR)表征产物结构, 并考察不同浓度氨基酸对中间产物碳酸钙和最终产物羟基磷灰石粉体及牙釉质基质的影响. 结果表明: 氨基酸浓度对溶液中游离晶体粉末的形貌影响较大, 由碳酸钙转化为羟基磷灰石, 具有形貌遗传性, 该影响未体现在牙釉质基质上; 吸附在牙釉质基质片的氨基酸作为矿化成核位点, 通过调节羟基磷灰石的晶体生长排列方向即可实现原位再矿化.

关键词: 釉质, L-丝氨酸(L-Ser), L-天门冬氨酸(L-Asp), 羟基磷灰石, 碳酸钙

Abstract:

Based on the principle of biomimetic synthesis, carbonized hydroxyapatite crystals were prepared by vapor diffusion method with L-serine(L-Ser) and L-aspartic(L-Asp) acted as an organic substrate, and bioinspired enamel repair was performed via L-Ser/Aspdirected assembly of hydroxyapatite
nanoparticles at the same time. The product was characterized by means of field emission scanning electron microscopy (FESEM), X-ray  diffraction (XRD) and Fourier transform infrared spectrometry (FTIR), and the effects of different concentrations of serine on the intermediates of calcium carbonate and final product of calcium hydroxyapatite limestone powder and dental film were studied. The results show that different concentrations of serine significantly affected the morphology of the free crystalline powder in solution, and the conversion of calcium carbonate to hydroxyapatite with morphological hereditary characteristics, as well as played an important role in the continuation of the crystal arrangement on the surface of enamel film.

Key words: enamel, L-serine (L-Ser), L-aspartic (L-Asp), hydroxyapatite (HA), calcium carbonate

中图分类号: 

  • O641