红车轴提取物对基质金属蛋白酶的抑制活性

红车轴提取物对基质金属蛋白酶的抑制活性

王书英¹,², 蒋坤², 杨金刚², 蒲利华³, 李聪¹, 申烨华¹, 房学迅²

1. 西北大学合成与天然功能分子化学教育部重点实验室, 新药研究所, 化学与材料科学学院, 西安 710069；2. 吉林大学分子酶学工程教育部重点实验室, 长春 130021； 3. 西安市儿童医院, 西安710003

收稿日期:2008-04-07 修回日期:1900-01-01 出版日期:2009-01-26 发布日期:2009-01-26
通讯作者: 房学迅

Inhibition of Trifolium Pratense Extract to Matrix Metalloproteinases Activity

WANG Shuying¹,², JIANG Kun², YANG Jingang², PU Lihua³, LI Cong¹, SHEN Yehua¹, FANG Xuexun²

1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Drug Discovery, College of Chemistry and Material Science, Northwest University, Xi’an 710069, China;2. Key Laboratory of Molecular Enzymology and Enzyme Engineering of the Ministry of Education, Jilin University,Changchun 130021, China; 3. Xi’an Children Hospital, Xi’an 710003, China

Received:2008-04-07 Revised:1900-01-01 Online:2009-01-26 Published:2009-01-26
Contact: FANG Xuexun

摘要/Abstract

摘要： 研究质量分数为40%的红车轴黄酮提取物及其含有的4种主要异黄酮对基质金属蛋白酶（MMP-16）的抑制作用. 结果表明, 质量分数为40%的红车轴黄酮提取物以剂量依赖的方式有效抑制MMP-16的活性, 刺芒柄素抑制MMP-16的活性效果较弱而且不是剂量依赖方式, 而鹰嘴豆芽素、大豆甙元和染料木黄酮不能抑制MMP-16的活性. 红车轴黄酮提取物通过色谱纯化, 得到33种纯化馏分, 其中13号馏分与25号馏分能抑制MM P-16的活性, 并且13号馏分的抑制作用是剂量依赖的.

关键词: 红车轴提取物, 基质金属蛋白酶-16, 基质金属蛋白酶抑制剂

Abstract: To study the inhibition mechanism of Trifolium pratense to tumor, we investigated the inhibitory activity of Trifolium pratense extract contain 40% flavonoids and four major isoflavones on MMP-16. Results show that the Trifolium pratense extract containing 40% flavonoids inhibits MMP-16 a ctivity, biochanin A, genistein and daidzein can’t inhibit MMP-16 activity, formon onetin can weakly inhibit MMP-16 activity but not dosedependent. After column chromatography analysis, we obtained 33 fractions, the No.13 fraction and No.25 fraction inhibit MMP-16 activity and the inhibitory effect is dosedependent for the No.13 fraction.

Key words: Trifolium pratense extract, MMP-16, MMPs inhibitor

中图分类号:

R285.5

王书英,, 蒋坤, 杨金刚, 蒲利华, 李聪, 申烨华, 房学迅. 红车轴提取物对基质金属蛋白酶的抑制活性[J]. J4, 2009, 47(01): 150-153.

WANG Shuying,, JIANG Kun, YANG Jingang, PU Lihua, LI Cong, SHEN Yehua, FANG Xuexun. Inhibition of Trifolium Pratense Extract to Matrix Metalloproteinases Activity[J]. J4, 2009, 47(01): 150-153.