利用十二肽库筛选心肌型脂肪酸结合蛋白特异性的亲和配体

利用十二肽库筛选心肌型脂肪酸结合蛋白特异性的亲和配体

张淑华¹,², 王立成¹, 王丽萍¹, 于源华², 李惟¹

1. 吉林大学生命科学学院, 长春 130021； 2. 长春理工大学生命科学技术学院, 长春 130022

收稿日期:2008-04-07 修回日期:1900-01-01 出版日期:2009-01-26 发布日期:2009-01-26
通讯作者: 王丽萍

Scanning Affinity Ligand of Hearttype Fatty Acid Bound Proteinwith Phagedisplayed 12mer Peptide Library

ZHANG Shuhua¹,², WANG Licheng¹, WANG Liping¹, YU Yuanhua², LI Wei¹

1. College of Life Science, Jilin University, Changchun 130021, China;2. School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China

Received:2008-04-07 Revised:1900-01-01 Online:2009-01-26 Published:2009-01-26
Contact: WANG Liping

摘要/Abstract

摘要： 采用噬菌体表面展示十二肽库对心肌型脂肪酸结合蛋白（HFABP）特异性亲和配体进行筛选, 经3轮筛选及序列比对后得到一个酶联免疫测定分析 (ELISA)检测阳性特征序列： WPNHHMLHKRWP. 对此序列进行肽合成, 并标记上荧光标记物芘, 经高效液相纯化、冻干及质谱确定其分子量后制成荧光肽探针检测急性心肌梗塞病人血样, 结果均呈阳性. 结果表明, 所获得的肽探针具有较好的临床应用效果.心肌型脂肪酸结合蛋白; 噬菌体表面展示十二肽库; 亲和配体; 酶联免疫测定分析

关键词: 心肌型脂肪酸结合蛋白, 噬菌体表面展示十二肽库, 亲和配体, 酶联免疫测定分析

Abstract: We obtained the specific sequence of affinity ligand of HFABP with phage-display 12mer peptide library. After three rounds of panning and sequences comparison, we obtained one ELISA positive sequence of WPN HHMLHKRWP. The target peptide was then synthesized by manual way. Treated by reverse phase HPLC (High Pressure Liquid Chromatography) and mass spectrometry, the target peptide coupled with pyrene was used as a fluorescent probe to detect HFABP in the samples of AMI patients. The experiment resultindicates that the samples of AMI patients are all positive. The result reflects that the target ligand peptide we got has shown a good effect on clinical application for AMI.

Key words: heart type fatty acid bound protein (HFABP), phage display 12mer peptide library, affinity ligand, enzyme-linked immuno sorbent assay (ELISA)

中图分类号:

Q513

张淑华,, 王立成, 王丽萍, 于源华, 李惟. 利用十二肽库筛选心肌型脂肪酸结合蛋白特异性的亲和配体[J]. J4, 2009, 47(01): 140-149.

ZHANG Shuhua,, WANG Licheng, WANG Liping, YU Yuanhua, LI Wei. Scanning Affinity Ligand of Hearttype Fatty Acid Bound Proteinwith Phagedisplayed 12mer Peptide Library[J]. J4, 2009, 47(01): 140-149.

[1]	郑明珠, 方丽, 吕萍, 陈亮, 刘景圣. 长白山林蛙输卵管糖蛋白的分离鉴定[J]. J4, 2011, 49(02): 336-339.
[2]	朴锦华, 李鸿梅, 王丽萍，高利增, 石玉华, 李惟. 胰岛素受体短肽配体的亲和筛选[J]. J4, 2003, 41(03): 384-387.