用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应

用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应

毕淑云¹,²，宋大千¹，魏士刚¹，王桂芬²，陈铁³，胡秀丽¹,⁴，刘忠英⁴，乔春玉¹，张寒琦¹

1．　吉林大学　化学学院，长春 130012； 2．　延边大学　理学院，吉林省延吉 133002; 3．　延边大学　分析测试中心，吉林省延吉 133002； 4．吉林大学　再生医学科学研究所，长春 130021

收稿日期:2005-09-08 修回日期:1900-01-01 出版日期:2006-05-26 发布日期:2006-05-26
通讯作者: 宋大千

Interaction between Indomethacin and Human Serum Albumin by Fluorescence Quenching Method

BI Shu-yun¹,², SONG Da-qian¹, WEI Shi-gang¹, WANG Gui-fen², CHEN Tie³, HU Xiu-li¹,⁴, LIU Zhong-ying⁴, QIAO Chun-yu¹, ZHANG Han-qi¹

1. College of Chemistry， Jilin University， Changchun 130012, China； 2. College of Science， Yanbian University， Yanji 133002, Jilin Province, China；3. Analysis and Instrumental Center， Yanbian University， Yanji 133002, Jilin Province, China； 4. Institute of Regenerative Medical Science， Jilin University， Changchun 130021, China

Received:2005-09-08 Revised:1900-01-01 Online:2006-05-26 Published:2006-05-26
Contact: SONG Da-qian

摘要/Abstract

摘要： 应用荧光光谱法研究了吲哚美辛与人血清白蛋白(HSA)的相互作用. 发现吲哚美辛对HSA的荧光有较强的猝灭作用. 采用两种计算结合常数K^A的方法，并对两种计算方法进行比较. 探讨了温度对吲哚美辛与HSA结合反应的影响，得到了不同温度下的结合常数 K^A 与结合位点数n值. 进一步探讨了一些金属离子对吲哚美辛与HSA结合常数的影响. 根据热力学常数确定了它们之间的主要作用力类型.

关键词: 吲哚美辛, 人血清白蛋白, 荧光光谱法

Abstract: The interaction between indomethacin and human serum albumin (HSA) was studied by means of the fluorescence quenching method. The binding constants were obtained at different temperatures. The effects of metal ions on the binding constants were discussed. According to the thermodynamic parameters， the main acting force between indomethacin and HSA was determined.

Key words: indomethacin, human serum albumin, fluorescence spectra method 

中图分类号:

O657

毕淑云,，宋大千，魏士刚，王桂芬，陈铁，胡秀丽,，刘忠英，乔春玉，张寒琦. 用荧光猝灭法研究人血清白蛋白与吲哚美辛的结合反应[J]. J4, 2006, 44(03): 493-496.

BI Shu-yun,, SONG Da-qian, WEI Shi-gang, WANG Gui-fen, CHEN Tie, HU Xiu-li,, LIU Zhong-ying, QIAO Chun-yu, ZHANG Han-qi. Interaction between Indomethacin and Human Serum Albumin by Fluorescence Quenching Method[J]. J4, 2006, 44(03): 493-496.

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