酶法提取百合多糖及其体外抗氧化活性

酶法提取百合多糖及其体外抗氧化活性

滕利荣，孟庆繁，刘培源，乔晓杭，王兆伏，贺秋华, 洪水声，陈佳，刘兰英

吉林大学生命科学学院，长春 130023

收稿日期:2003-06-09 修回日期:1900-01-01 出版日期:2003-10-26 发布日期:2003-10-26
通讯作者: 刘兰英

Extracting Lilium Brownii Polysaccharide by Combined-enzymes and Its Antioxidative Activity in Vitro

TENG Li-rong, MENG Qing-fan, LIU Pei-yuan, QIAO Xiao-hang, WANG Zhao-fu , HE Qiu-hua, HONG Shui-sheng, CHEN Jia, LIU Lan-ying

College of Life Science, Jilin University, Changchun 130023, China

Received:2003-06-09 Revised:1900-01-01 Online:2003-10-26 Published:2003-10-26
Contact: LIU Lan-ying

摘要/Abstract

摘要： 研究了热水法及复合酶法提取百合多糖（LBP）的最佳条件，对两种方法得到的多糖的生物活性进行了比较分析. 发现复合酶法能显著提高百合多糖的提取率，可达31.03% ，是热水法的2.85倍. 复合酶法提取的多糖在体外抗氧化活性如对O^－₂·及·OH的抑制作用、对H₂O₂诱导的人血红细胞氧化溶血的抑制作用及人红细胞膜脂质过氧化的抑制作用均显著高于热水法提取的多糖. 经Sephadex G-75柱层析对粗多糖进行分离，得到3种组分，其中活性组分LBP-3主要存在于酶法提取的多糖中, 测得该活性组分的分子量为13 400.

关键词: 百合多糖, 复合酶法, 抗氧化, LBP-3

Abstract: Two methods of extracting Lilium brownii polysaccharide, hot-water method and combined-enzyme method, were studied and the optimum conditions of t he two methods were found. The yield of the polysaccharides extracted by combine d　enzymes was 2.8 times that of polysaccharides extracted by hot-water. It was also found that the antioxidative activity of the polysaccharides, such as i nhibiting effects on O^-₂·, ·OH, the hemolysis of human R BC induced by H₂O₂ and the formation of MDA, extract ed by combined-enzymes were much greater than that of those extracted by hot-wa ter. After extracted by the two methods, the polysaccharides were separated by s ephedex G-75 gel filtration chromotography and three fractions were eluted out. LBP-3, whose molecular weight was about 13 400 and had the highest antioxida tive activity of the three fractions, was found to exist in polysaccharides extr ated by combined　enzymes much more than that to exist in those extracted by hot-water.

Key words: Lilium brownii polysaccharide, combined-enzyme method, antioxide, LBP-3

中图分类号:

Q539

滕利荣，孟庆繁，刘培源，乔晓杭，王兆伏，贺秋华, 洪水声，陈佳，刘兰英. 酶法提取百合多糖及其体外抗氧化活性[J]. J4, 2003, 41(04): 538-542.

TENG Li-rong, MENG Qing-fan, LIU Pei-yuan, QIAO Xiao-hang, WANG Zhao-fu , HE Qiu-hua, HONG Shui-sheng, CHEN Jia, LIU Lan-ying. Extracting Lilium Brownii Polysaccharide by Combined-enzymes and Its Antioxidative Activity in Vitro[J]. J4, 2003, 41(04): 538-542.

[1]	张蕾, 李秋双, 郝婧玮, 宛春雷, 景云荣, 柴军红, 纪秋妍. 高效液相色谱法检测紫苏梗提取物活性成分及抗氧化效果[J]. 吉林大学学报(理学版), 2020, 58(4): 1020-1025.
[2]	王朋朋, 徐佳, 张丽梅, 张淑华, 王丽萍. 5种药食同源植物的抗氧化作用[J]. 吉林大学学报(理学版), 2018, 56(4): 1035-1039.
[3]	王丽萍, 崔雅欣, 徐佳, 侯雨菲, 吐伦阿依·艾合买提, 王嘉琪, 王立成. 余甘子对秀丽隐杆线虫的抗衰老作用[J]. 吉林大学学报(理学版), 2018, 56(3): 738-742.
[4]	王丽萍, 张丽梅, 侯思远, 徐佳, 隋天卓, 张瑞宁, 王琪菲, 王立成. 以秀丽隐杆线虫为模型的玛咖功效评价[J]. 吉林大学学报(理学版), 2018, 56(2): 440-445.
[5]	徐佳, 张丽梅, 王立成, 隋天卓, 王琪菲, 王丽萍. 松花粉的抗氧化作用[J]. 吉林大学学报(理学版), 2017, 55(06): 1626-1630.
[6]	张丽梅, 徐佳, 周潭, 张越, 王丽萍, 王立成. 葛根中黄酮提取工艺的优化及抗氧化作用[J]. 吉林大学学报(理学版), 2017, 55(04): 1020-1024.
[7]	李维佳，魏景艳，孙晔，牟颖，吕绍武，闫岗林，罗贵民. 具有GPX活性单链抗体的制备及其抗氧化效应[J]. J4, 2004, 42(03): 458-462.
[8]	梅子青，王海晶，尹兰，王丰，朴真三. 枝动菌对变压器介质油及其抗氧化剂代谢的初步研究[J]. J4, 2003, 41(01): 123-125.