亲和毛细管电泳法测定3种黄酮类化合物与人血清白蛋白的结合常数

Determination of Binding Constant between Human Serum Albumin and Flavonoid Compounds by Affinity Capillary Electrophoresis

ZHOU Xin¹,WANG Zi-ming¹,ZOU Ming-qiang²,ZHENG Jing¹,SONG Da-qian¹,ZHENG Jian¹,WANG Lu¹,ZHANG Han-qi¹

(1． Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China;　2． Institute of Inspection Technology and Equipment, Chinese Academy of Inspection and Quarantine, Beijing 100025, China)

Received:2005-01-21 Revised:1900-01-01 Online:2005-09-26 Published:2005-09-26
Contact: ZHANG Han-qi

Abstract

Abstract: The binding constants between human serum albumin (HSA) and each of three flavonoid compounds were determined by affinity capillary electrophoresis (ACE). The binding constants are 9.76×10⁵mol／L, 1.24×10⁶mol／L and 3.79×10⁵ mol／L for luteolin, quercetin and kaempferol, respectively. The results show that ACE is a useful method to study the interaction between drug and protein.

Key words: affinity capillary electrophoresis, luteolin, quercetin, kaempferol, human serum albumin, binding constant

CLC Number:

O657.8

ZHOU Xin,WANG Zi-ming,ZOU Ming-qiang,ZHENG Jing,SONG Da-qian,ZHENG Jian,WANG Lu,ZHANG Han-qi. Determination of Binding Constant between Human Serum Albumin and Flavonoid Compounds by Affinity Capillary Electrophoresis[J].J4, 2005, 43(05): 669-672.

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Determination of Binding Constant between Human Serum Albumin and Flavonoid Compounds by Affinity Capillary Electrophoresis

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