Abstract: Escherichia coli phage  vB_EcoMep3 detected in contaminated water was selected as a model virus, and the indoor dynamic migration simulation experiment  was carried out. We analyzed the migration process of nanoscale pathogenic microorganism—virum in groundwater by using DLVO theory and colloid filtration theory, and investigated the migration regularity of enterovirus colloid under different groundwater acidbase environments. The results show that: When the groundwater environment changs from acidic condition （pH=5.0） to alkaline condition （pH=9.0）, the particle sizes of virus colloid decrease and the mass recovery rates increase, which are 125%,291% and 618%, respectively; the coefficients of deposition rate decrease, which are 41×10^-4,28×10^-4 and 1×10^-4, respectively; Zeta potential decreases, while DLVO barrier and the stability of the virus increase; the ability of the virus colloid to deposit on the surface of the lithology medium weakens.

Key words: enterovirus, biocolloid, acidbase environment, migration regularity, groundwater