双液相反应体系中脂肪酶催化拆分缩水甘油酯

Kinetic Resolution of Glycidyl Esters Catalyzed by Lipase in a Biphasic Reaction System

WANG Zhi¹, QUAN Jing¹, WENG Liang¹, GOU Xiao -jun², MA Ji-sheng¹, ZHANG Gui-rong^1,3 , CAO Shu-gui¹

1. Key Laboratory for Molecular Enzymology and Engineering of Educational Ministry, Jilin University, Changchun 130023, China; 2. Key Laboratory of Molecular Evolution and Engineering, Chengdu University, Chengdu 610106, China;3. Educational Institute of Changchun, Changchun 130061, China

Received:2002-11-06 Revised:1900-01-01 Online:2003-04-26 Published:2003-04-26
Contact: CAO Shu-gui

Abstract

Abstract: The stereoselective hydrolysis of racemic glycidyl esters catalyzed by lipase in a biphasic system was studied. The results show that porcine pancreatic lipase (PPL) shows a high enantioselectivity towards glycidyl butyrate. The results also show (1) the optimal pH value was 7.6, (2) the optimal reaction t emperature was 27 ℃ and (3) the optimal reaction time was 4 h. Under all above optimal conditions, R-glycidyl butyrate with more than 93.3%　ee was pre pared and the hydrolysis degree of racemic glycidyl butyrate was 60.6%.

Key words: glycidyl ester, resolution, lipase, hydrolysis

CLC Number:

WANG Zhi, QUAN Jing, WENG Liang, GOU Xiao -jun, MA Ji-sheng, ZHANG Gui-rong , CAO Shu-gui. Kinetic Resolution of Glycidyl Esters Catalyzed by Lipase in a Biphasic Reaction System[J].J4, 2003, 41(02): 213-216.

[1]	YU Shengbao, FANG Yu, GAO Lihui, DIAO Shu. SHEPWM Control Method of Controllable Emission Current Frequency Based on Improved Particle Swarm Optimization Algorithm [J]. Journal of Jilin University Science Edition, 2020, 58(5): 1207-1214.
[2]	GUO Baosheng, YANG Yiyan, HU Junhan, HAN Weiwei, LI Wannan. Preparation of Small Molecular Peptides from Sunflower Calathideby Enzymatic Hydrolysis Method Using Response Surface Method#br# [J]. Journal of Jilin University Science Edition, 2019, 57(5): 1275-1280.
[3]	WU Zhuofu, LI Meng, ZHANG Sitong, CHEN Guang. Preparation of Mung Bean Polypeptide by Hydrolysis of Papain and Alcalase [J]. Journal of Jilin University Science Edition, 2018, 56(4): 1029-1034.
[4]	DONG Liyan, ZHANG Gaoxiang, SUN Bo, LANG Yining. Ambiguity Resolution Algorithm for Mapping from Natural Language to SPARQL Languege [J]. Journal of Jilin University Science Edition, 2016, 54(03): 535-538.
[5]	LIU Lei, ZHANG Zijia, LIU Lei, ZHANG Rui. A Method of GPUBased Accelerating 2DMulti-resolutions Discrete Wavelet Transform [J]. Journal of Jilin University Science Edition, 2015, 53(02): 267-272.
[6]	. TwoDimensional Wavelet with Tectonic Scale a and FourDirection [J]. Journal of Jilin University Science Edition, 2013, 51(05): 763-782.
[7]	FAN Mu-Jie, FANG Shao-Jun, LI Xiao-Meng. Application of ADI\\|MRTD Method on New ACPW Filter [J]. J4, 2011, 49(06): 1115-1118.
[8]	WANG Jia-Xin, SUN Re-Xi, XUN Er-Na, ZHANG Ai-Jun, WANG Lin, WEI Xiao-Fei, WANG Lei, WANG Zhi. UltrasoundPromoted Resolution of(R,S)Glycidyl Butyrate Catalyzed by Lipase [J]. J4, 2011, 49(04): 782-786.
[9]	DIAO Shuang, YANG Li-Juan, TUN Jia-Zhen, LIN Fan, FANG Hua-Xun, WANG En-Sai. Preparation of S(+)3-Methyl-1-(2-(1-piperidinyl)phenyl)butylamine through Enzymatic Catalysis [J]. J4, 2010, 48(06): 1043-1046.
[10]	CUI Lin-Lin, ZHANG Qun-Li, MI Gao-Yu, WANG En-Sai. Synthesis of 7-Substituted-2-amino-1,2,3,4-tetrahydro-2-naphthalene Carboxylic Acid [J]. J4, 2010, 48(05): 860-864.
[11]	LIANG Hua-Zhang, XUE Yao-Gong, LI Jiang. Application of Loop Subdivision Wavelet Frames tothe Compression of 3D Graphics [J]. J4, 2009, 47(05): 986-993.
[12]	ZHANG Guoyan,, CAO Shugui. The Newly Development of theEnzymecatalyzed Resolution Methods and Application [J]. J4, 2008, 46(05): 997-1000.
[13]	ZHAO Shuang, XU Zhibing, E Chenguang, LI Zhuo, WANG Ensi,. Synthesis of Antidiabetic Medicine Repaglinide [J]. J4, 2008, 46(03): 556-559.
[14]	WANG Hongzhi, ZHAO Shuang, HU Yanjun. Superresolution Image Restoration with MAP Based on L-Curve [J]. J4, 2008, 46(02): 275-278.
[15]	CHENG Yueming, WANG Lei, YU Dahai, GAO Feifei， CHEN Xiang, ZHANG Shudi, CAO Shugui, WANG Zhi. Preparation of Glycidol by Combination of Enzymatic and Chemical Methods [J]. J4, 2007, 45(04): 681-685.

Kinetic Resolution of Glycidyl Esters Catalyzed by Lipase in a Biphasic Reaction System

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0