利用酸值监测脂肪酶催化豆油水解反应的进程

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Lipase-catalyzed Soybean Oil Hydrolysis Monitored via Acid Number Detection

GAO Gui¹, ZHENG Liang-yu¹, HAN Si-ping¹, ZHANG Zuo-ming¹, LI Shi-jun², CAO Shu-gui¹

(1. Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130021, China； 2. Changchun Changsheng Gene Pharmaceutical Limited Company, Changchun 130012, China)

Received:2005-07-05 Revised:1900-01-01 Online:2005-11-26 Published:2005-11-26
Contact: CAO Shu-gui

Abstract

Abstract: Hydrolysis of soybean oil catalyzed by lipase from Candida lipalytica was investigated. The relative concentrations of the components in the hydrolysate were determined by means of HPLC, furthermore, a new rapid method to monitor soybean oil hydrolytic process was developed based on the relationship between the acid number and the relative concentration of the various components in the hydrolysate.

Key words: lipase, hydrolysis, HPLC, glyceride, acid number

CLC Number:

Q55

GAO Gui, ZHENG Liang-yu, HAN Si-ping, ZHANG Zuo-ming, LI Shi-jun, CAO Shu-gui. Lipase-catalyzed Soybean Oil Hydrolysis Monitored via Acid Number Detection[J].J4, 2005, 43(06): 853-857.

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Lipase-catalyzed Soybean Oil Hydrolysis Monitored via Acid Number Detection

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