Abstract: Thermostable Tgo DNA polymerase is obtained from Thermococcus gorgonarius by the molecular cloning and express technology. Plasmid19, wild barley Na+/H⁺ reverse transporter gene, mous Cx37 gene and human CYT2C9 gene were successfully amplified with recombinase. The amplification capability and amplification fidelity of the enzyme were compared with those of Taq enzyme. The result indicates that the amplification capability of Tgo enzyme is the same as that of Taq enzyme. But the amplification fidelity of Tgo enzyme is over 30 times higher than that of Taqenzyme and 1.6 times higher than that of Pfu.

Key words: Tgo DNA polymerase, amplification capability, amplification fidelity