Abstract:

We peeled fresh Aloe chinensis Baker, extracted and diluted with PBS buffer. The nematodes were cultured on nematode growth medium containing with different mass concentrations of D-glucose, which was used to evaluate the optimal mass concentration by conventional physiological index such as lifespan, athletic ability of nematodes. And the effect of aloe extract on reducing fat accumulation was showed by oil red O staining and Image J software analysis. Experimental results show that the 2%(2 gD-glu/100 mL NGM) is the optimal induced mass concentration to establish the Caenorhabditis elegans fat accumulation model, and aloe extract has excellent effect on reducing fat accumulation model of C. elegans.

Key words: Caenorhabditis elegans, induced by D-galactose, fat accumulation, Aloe chinensis  Baker extract