Abstract:

An RPHPLCDAD method was established for the determination of 5,6,7,3′,4′pentamethoxyflavone and 5,7,8,3′,4′pentamethoxyflavone. An Acchrom Unitary C18 (4.6 mm×250 mm,5 μm)Column was used. The mobile phase consisted of V(methanol)∶V(water)=52∶48, at a flow rate of 1.0 mL/min. The column temperature was 25.0 ℃ and the wavelength of DAD detector was set at 310 nm. The linear range of 5,6,7,3′,4′pentamethoxyflavone was 0.011—5.3 μg(r=0.999 9) and the mean recovery rate was 101.40%(RSD=1.22%), while the linear range of 5,7,8,3′,4′pentamethoxyflavone was 0.010—5.1 μg(r=0.999 9) and the mean recovery rate was 100.70% (RSD=1.51%). The mass ratios of two flaronoids were 0.094,0.015 mg/g respectively in Fructus aurantii-come from Hebei Anguo, while they were 0.22,0.032 mg/g respectively come from Anhui Bozhou.

Key words: Fructus aurantii, HPLC method, 5,6,7,3′,4′-pentamethoxyflavone, 5,7,8,3′,4′-pentamethoxyflavone, mass ratio