Abstract: A strain pdi which can use biphenyl as the sole carbon source was isolated from seabed mud and cultured for 7 dwith 150 mL of fluid medium which contained 7.5 mg/L polychlorinated biphenyls (PCBs).　GCMS measurement showed PCBs degradation efficiency was 95.38%. The genome was amplified with bacterium 16SrDNA primer. The amplified product (about 1 500 bp) was purified and sequenced for phylogenetic analyses with Genbank. The bacterium pdi was identified as Pseudomonsa spTS1138. RAPD analyses of pdi produced stable DNA fingerprint. These studies have provided parameter for the further dynamic resolution of bacterium clusters for the rehabilitation of PCBs contaminated underground water environment with pdi PRB method.

Key words: PCBs, 16SrDNA, biodegradation, phylogenetic, RAPD