J4 ›› 2010, Vol. 48 ›› Issue (06): 1070-.
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LI Jianhui, LU Panpan, ZHANG Yaping
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16S rDNA V3 fragmentbase was separated by DGGE (denaturing gradient gel electrophoresis) and clone sequence BLAST technologies, and quantity one software was used to map the cluster analysis. The fingerprint shows that the compositions of bacteria in all the soil samples are obvriously different. The cluster analysis indicates that the structures of bacteria in the same month of soil samples in different depths are similar to each other. Specific band sequencing results show that the Pseudomonas sp is the dominant microorganisms in mud volcanic soils. The bacterial polymorphism in mud volcano soil in Xinjiang is significant, and this diversity is vulnerable to ecological, climatic and other factors, however, these factors affect the dominant microorganisms little.
Key words: mud volcano, 16S rDNA, denaturing gradient gel electrophoresis(DGGE), DNA fingerprinting
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LI Jian-Hui, LU Fen-Fen, ZHANG E-Beng. Analysis of the Bacterial Communities in Mud Volcano Soilsin Xinjiang via DGGE[J].J4, 2010, 48(06): 1070-.
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