Abstract:

Taking E.coli as the host, we expressed a recombinant protein of Yersinia pestisouter protein H(ΔYopH), which contained the catalytic domain of protein tyrosine phosphatase YopH. After purification, with the ΔYopH as the target, we studied inhibitory effect from 111 kinds of traditional Chinese medicine (TCM) aqueous extracts via an \%in vitro\% enzymatic reaction kinetics experiment, and found three TCMs had obvious inhibitory effect. They were gallnut, sanguisorba and pomegranate peel. Further more, we analyzed their median inhibitory concentration (IC\-\{50\}) values and inhibitory types.

Key words: Yersinia pestisouter protein H (YopH), protein tyrosine phosphatase, traditional Chinese herb, inhibitor