Abstract:

To construct the short hairpin RNA (shRNA) interference expression plasmid vectors of human HER2/neu gene, the recombinant plasmid was identified by PCR coupled with sequencing. The vector was transfected into SK-BR-3 cells, HER2/neu mRNA, protein, and the cell migration were assayed.
 The result indicated that the construction of recombinant plasmid was successful. Transfection of shRNA plasmid significantly downregulated HER2/neu mRNA and protein; at the same time, the migration ability of SKBR3 cell was obviously inhibited. Recombinant plasmid 1 had the strongest effect, with the inhibition ratios of 84.65% at the mRNA level and 74.98% at the protein level.

Key words: HER2/neu; breast cancer, shRNA interfering vector