Abstract:

The reductive amination method was used for the fluorescently labelling of Ganoderma applanatum polysaccharide(GAP). FITC was linked up with the reducibility termination of GAP, and fluorescent substitute ratio was determined to be 090%. The results of the measurement by xCELLigence RTCA DP automatic realtime cell analyzer show in vitro the cytotoxic activity did not significantly change before and after fluorescent labeling of GAP. The results measured by means of flow cytometer and fluorescence microscope show fluorescence signal was detected both on the cell membrane and in the nucleus, illustrating that GAP can be attached to human colorectal cancer SWWC1116 membrane, and can be further transported to the nuclei.

Key words: Ganoderma applanatum, polysaccharide, fluorescent labelling, human colorectal cancer SWWC1116, cellular localization