Abstract:

We constructed a fusion gene consisted of five copies longacting Glucagonlike peptide1 (rolGLP-1) and Hirudin (rHV) called 5rolGLP-HV by molecular cloning. 5rolGLP=HV gene was cloned into the expression vector pET22b (+) and translated into BL21 (DE3), and a highly expression bacterial strain was obtained. The expression product 5rolGLPHV was analyzed by SDS-PAGE electrophoresis and Western bolt, and was purified with NiNTA. Function analysis was performed via STZinduced type Ⅱ diabetic mice and thrombosis mice. The results show 5rolGLPHV has reducing blood glucose and antithrombotic effects.

Key words: diabetes mellitus, thrombosis, 5rolGLPHV; expression, purification, function analysis