J4 ›› 2012, Vol. 50 ›› Issue (03): 581-.
Previous Articles Next Articles
WU Congmei, ZHAO Yunhui, LI Yingying, SUN Baowa, YIN Yuhe
Received:
Online:
Published:
Contact:
Abstract:
Using the computer simulation technology of docking and the phage peptide library for optimum screening of the isocitrate lyase peptide inhibitor. First the binding peptides were screened by phage peptide library, which has a high affinity for isocitrate lyase(ICL). And then via the Discovery Studio 21, the docking of peptides to ICL protein crystal (1F8I) was simulated. Finally, the solidphase synthesis of Fmoc was used to synthesize peptide, and their biological activities were detected. We got 29 heptadpeptide sequences by phage peptide library screening, 12 of which can successfully dock to ICL protein crystal. The biological activity detecting shows that they all have conspicuous inhibition on the ICL activity (rate of inhibition 50%).
Key words: isocitrate lyase, peptide inhibitor, phage peptide library, virtual screening, docking
CLC Number:
Tun-Cong-Mei, DIAO Wen-Hui, LI Ying-Ying, SUN Bao-Wa, YAN Yu-He. Optimization Screening of Isocitrate Lyase Peptide Inhibitor[J].J4, 2012, 50(03): 581-.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/lxb/EN/
http://xuebao.jlu.edu.cn/lxb/EN/Y2012/V50/I03/581
Cited