Abstract:

In order to get HIVGAG pharmaceutical grade plasmid DNA vaccine, we studied  the procedure of the cultivation of bacteria, alkaline lysis, isolate rudely extraction and chromatography to get plasmid DNA. We used hollow fiber ultrafiltration concentration, ion exchange chromatography and composite model gel filtration medium in this study. The experimental results show that our final product meets the requirments of  pharmaceutical standardization. And
 this technology is suit for largescale production of DNA vaccines.

Key words:  DNA vaccine, alkaline lysis, ion exchange chromatography, composite model gel filtration medium