Abstract: The hypoglycemic active ingredient in the ethyl acetate layer of rhizome of Acanthopanax senticosus was separated and purified by normal phase silica gel column chromatography, C-18 reverse phase column chromatography and semipreparative high performance liquid chromatography (HPLC). Ten compounds were obtained, and the structures of the obtai
ned compounds were determined by analyzing spectral data of nuclear magnetic resonance hydrogen spectroscopy (1HNMR), nuclear magnetic resonance carbon spectroscopy (13C
NMR), distortionless enhancement by polarization transfer (DEPT), mass spect
rum (MS), and compared with the relevant literatures. Their activities were tested. The results show that compounds 〖STHZ〗1
〖ST〗—〖STHZ〗5〖ST〗 are kauranetype diterpenoids, compounds 〖STHZ〗6〖ST
〗—〖STHZ〗8〖ST〗 are turpentinetype diterpenoids, and compounds 〖STHZ〗9〖
ST〗,〖STHZ〗10〖ST〗 are lignin compounds. Compounds 〖STHZ〗1〖ST〗,〖STHZ〗5〖ST〗,〖STHZ〗7〖ST〗,〖STHZ〗8〖ST〗 h
ave significant specific inhibitory activity on protein tyrosine phosphatase 1B (PTP1B), 
compounds 〖STHZ〗2〖ST〗,〖STHZ〗4〖ST〗,〖STHZ〗9〖ST〗 have  good inhibitory effects on 
protein phosphatase 1 (PP1), and compounds 〖STHZ〗9〖ST〗,〖STHZ〗10〖ST〗 have a low inhibitory effect on
 vaccinia H1 related (VHR) phosphatase.

Key words: Acanthopanax senticosus, active ingredient, protein , tyrosine phosphatase 1B (PTP1B), diabetes