Journal of Jilin University Science Edition ›› 2020, Vol. 58 ›› Issue (3): 704-710.

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Differential Expression Analysis between Transcriptome and Proteome of StNPS6 Gene Deletion Mutant of Setosphaeria turcica

LI Wanze1, LIU Yang1, ZHANG Yanhua2, LIU Jinliang2, PAN Hongyu2, ZHANG Xianghui2   

  1. 1. College of Agriculture, Jilin Agriculture Science and Technology College, Jilin 132001, Jilin Province, China；2. College of Plant Science, Jilin University, Changchun 130012, China
  • Received:2019-08-20 Online:2020-05-26 Published:2020-05-22
  • Contact: ZHANG Xianghui E-mail:zhangxianghui1982@126.com

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Abstract: We used TDNA insertion method to construct a mutant library of Setosphaeria turcica, and screened a mutant with significantly decreased pathogenicity. T-DNA insertion site of the mutant was upstream promoter region of the nonribosomal peptide synthetases 6 (StNPS6) gene. We first knocked out the StNPS6 gene, and then analyzed the differential expression analysis of transcriptome and proteome of StNPS6 gene knockout mutants and wild types. The results show that  the 903 genes are upregulated and 864 genes are downregulated
among 1 767 differentially expressed genes. The 8 proteins are upregulated and 22 proteins are downregulated among 30 differentially expressed proteins identified in the mutant.

Key words: Setosphaeria turcica, StNPS6 gene, transcriptome, proteome

CLC Number: 

  • Q933
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