Abstract: The electrochemical detection method based on graphene oxide quantum dots/multi walled carbon nanotubes/screen printed electrode (GOQDs/MWCNTs/SPCE*) was used to simultaneously detect thymine and cytosine in BALB/c 3T3 cells. The effects of enrichment potential, enrichment time and pH values on the electrochemical behavior of thymine and cytosine were investigated. The experimental results show that   the optimal detection conditions are as follows：  enrichment potential  is 0, enrichment time is 150 s, pH=7.4.  The detection limits of  thymine and cytosine are 0.69,0.95 μmol/L, respectively. The electrochemical detection method can be used to  detect thymine and cytosine in BALB/c 3T3 cells simultaneously.

Key words: electrochemistry; , cell; , thymine; , cytosine, simultaneously detect