Abstract: Based on the fact that glioblastoma multiforme is the most common malignant tumor in the brain,  an average survival time of  patients is relatively short,  and traditional treatment methods are difficult to achieve  cure. However, emerging oncolytic virotherapy has limited dissemination capability and  suboptimal treatment effects. By using genetic engineering technology to modify the capsid protein of human adenovirus type 5 (Ad5), we constructed a chimeric oncolytic adenovirus with a fiber knob replacement to enhance its ability to infect and target  glioma cells. By replacing the  knob of Ad5 with the knob  of Ad37 from subgroup B Ad37, it was confirmed that it could utilize sialic acid as a receptor, thereby improving its ability to infect and kill tumor cells with low expression of  CAR (coxsackievirus and adenovirus receptor) such as glioma, and improving its penetration efficiency through the blood\|brain barrier in in vitro models,  providing a new solution for the treatment of glioblastoma multiforme.

Key words: glioblastoma multiforme, oncolytic adenovirus, genetic engineering