Abstract: We studied the  molecular mechanism of antioxidative activity of stamen nelumbinis   by network pharmacology and molecular docking technology,  and  verified the free radical scavenging ability of stamen nelumbinis  through  in vitro experiments. The results show  that the antioxidant effects of stamen nelumbinis   mainly relies on the regulation of biological processes such as MAPKs signaling pathway and PI3K/AKT signaling pathway. The stamen nelumbinis  has a certain ability to clear 1,1-diphenyl-2-picrylhydrazyl radical (DPPH^.) and hydroxyl radical (^.OH),  and the scavenging rates of DPPH^. and ^.OH are 83.6% and 53.43%,  respectively when the mass concentration is 0.8 mg/L. The high performance liquid chromatography (HPLC) detection reveals that the stamen nelumbinis  contains flavonoids such as kaempferol,  lignans,  and quercetin, which exert antioxidant effects.

Key words: stamen nelumbinis,  , antioxidant,  , bacteriostatic agent,  , network pharmacology,  , molecular docking,  , high performance liquid chromatography analysis