Abstract: Rana dybowskii residue was used as raw material， the  Rana dybowskii residue peptides (RDRP) was abtained  under enzymatic hydrolysis conditions, and then  were chelated with selenium ions (Se⁴⁺) to prepare  Rana dybowskii residue peptide chelated selenium (RDRPCS). We characterized the synthesized compounds, studied the optimized conditions for chelation reaction, and investigated their anti-inflammatory activity through in vitro cell models. The experimental results show that  Se⁴⁺ reacts with RDRP to form chelates.  RDRPCS has sufficient metal binding sites,  with chelating sites being  amino,  hydroxyl,  and C=O.    The optimization result of chelation process is that temperature is 60  ℃,   pH=6,  time is 65 min,  and a mass ratio is 2.5∶1， the efficiency of chelation reaction under this conditions is 70.7%.  Different concentrations of chelates have inhibitory effects on the release of  NO, TNF-α,IL-6 and IL-1β in RAW264.7 inflammatory cell model.

Key words: Rana dybowskii residues peptides,  , chelating selenium,  , anti-inflammatory, cell model