应用于生物检测的微流体聚焦芯片的研制

J4 ›› 2011, Vol. 29 ›› Issue (01): 31-.

应用于生物检测的微流体聚焦芯片的研制

吴元庆|姚素英|高鹏

天津大学电子信息工程学院|天津 300072

出版日期:2011-03-25 发布日期:2011-03-04
通讯作者: 吴元庆（1982— ），男，辽宁庄河人，天津大学博士研究生，主要从事微电子与固体电子学研究，(Tel)86-18920366825 E-mail:wuyuanqing123@163.com
作者简介:吴元庆（1982— ）|男|辽宁庄河人|天津大学博士研究生|主要从事微电子与固体电子学研究|(Tel)86-18920366825 (E-mail) wuyuanqing123@163.com；姚素英（1947— ）|女|天津人|天津大学教授|主要从事微电子学与固体电子学研究（Tel）86-22-27890832(E-mail)syyao@tju.edu.cn。
基金资助:
国家自然科学基金资助项目（07JC2DJC10400）

Fabrication of Microfluidic Focusing Chip for Bioinstrumentation

WU Yuan-qing|YAO Su-ying|GAO Peng

School of Electronic and Information Engineering, Tianjin University, Tianjin 300072, China

Online:2011-03-25 Published:2011-03-04

摘要/Abstract

摘要：

为了完成生物检测实验中待检测微球的单列通过，基于流式细胞仪的技术原理，设计了一种微流体聚焦芯片，并利用Intellisuite软件对聚焦进行仿真和设计。用微浇铸方法制作基于聚二甲基硅氧烷的微流控芯片，并采用插针式封接，提高了芯片成品率。通过实验制作芯片，并将它应用于测试，成功地实现了直径为10 μm的悬浮待检测微球呈单列顺序排列。

关键词: 聚焦, 微流控芯片, 生物检测

Abstract:

In order to make the suspended microspheres to be detected separately and monostichously, based on the technical principles of flow cytometry, a kind of microfluidic focusing chip was designed. The focusing chip was simulated and designed by using software Intellisuite. This PDMSbased microfluidic chip was produced by casting method, adopting a sealing method as contact pins, which greatly improved the yield of chips. Through the experiment, the microfludic focusing chip is fabricated, and is applied to the testing realizing the suspended 10 μm diameter microspheres ordered separate and monostichous.

Key words: focusing, microfluidic chip, biological detection

中图分类号:

TH79

吴元庆|姚素英|高鹏. 应用于生物检测的微流体聚焦芯片的研制[J]. J4, 2011, 29(01): 31-.

WU Yuan-qing|YAO Su-ying|GAO Peng. Fabrication of Microfluidic Focusing Chip for Bioinstrumentation[J]. J4, 2011, 29(01): 31-.