Abstract: Rapidity and accuracy are most important in medical treatment. Traditional blood tests rely on experienced physicians, which leads to low efficiency and accuracy. For the first time, a comprehensive determination method based on image recognition technology, named P-HSV ( Perimeter-Hue, Saturation, Value), is proposed for microfluidic blood sample chips. Size and color are used for integrated interpretation of blood types. Size interpretation is based on the contour perimeter and number of agglutination clusters within the reaction chamber, while color interpretation is based on categorization of the color saturation (S: Saturation) to brightness ( V: Value ) ratio of agglutination clusters within the reaction chamber. The grade of blood agglutination reaction is synthetically determined by size and color results. In this method, machine vision is used to determine the grade of blood agglutination reaction, resulting in accurate and rapid blood type determination. This reduces the subjective judgment of artificial judgment, improving the detection speed and accuracy greatly.

Key words: blood types, image interpretation, contour perimeter, hue, saturation, value(HSV) color model