基于有机-无机杂化材料的硝酸还原酶电极的研究

基于有机-无机杂化材料的硝酸还原酶电极的研究

赵林，于洋，闫博，谭欣，李若璞

天津大学环境科学与工程学院，天津 300072

收稿日期:2006-10-20 修回日期:1900-01-01 出版日期:2007-03-26 发布日期:2007-03-26
通讯作者: 赵林

Amperometric Nitrate Reductase Biosensors Based on Organic-inorganic Hybrid Material

ZHAO Lin, YU Yang, YAN Bo, TAN Xin, LI Ruo-pu

School of Environmental Science and Technology，Tianjin University，Tianjin 300072, China

Received:2006-10-20 Revised:1900-01-01 Online:2007-03-26 Published:2007-03-26
Contact: ZHAO Lin

摘要/Abstract

摘要： 在制备由聚乙烯醇和二氧化硅溶胶凝胶掺杂而成的有机-无机杂化材料的基础上，将硝酸还原酶用此杂化材料包埋固定在金盘电极上，利用甲基紫精为电子媒介体，制得高性能的安培型硝酸还原酶电极。此酶电极可以在-0.68 V（SCE）下对硝酸根进行测定，最适pH值为7.0，载酶量为7.2 mU。在选定的工作条件下，此酶电极的灵敏度和线性范围分别为2.53 nA/(μmol·L^-1)和0.01～0.36 mmol/L ，平均响应时间为12.1 s左右。

关键词: 硝酸还原酶传感器, 修饰电极, 甲基紫精, 溶胶-凝胶, 聚乙烯醇, 杂化材料

Abstract: An organic-inorganic hybrid material based on sol-gel derived SiO₂ and PVA has been obtained. Nitrate reductase is immobilized with this hybrid material on gold electrode surface and an amperometric nitrate reductase biosensor of high performance has been fabricated. The nitrate reduction reaction is driven by the enzyme and methyl viologen which is used as the electrontransfer mediator. The developed sensor is applied to detect nitrate with operating potential of -0.68V (vs. SCE). Optimum pH is 7.0 and enzymes loading are 7.2 mU. Under selective conditions the biosensor shows sensitivity of 2.53 nA/(μmol·L^-1) and a linear range of 0.01～0.36 mmol/L. The average response time is 12.1 s.

Key words: nitrate reductase sensor, modified electrode, methyl viologen, sol-gel, PVA, hybrid material

中图分类号:

O657.1

赵林，于洋，闫博，谭欣，李若璞. 基于有机-无机杂化材料的硝酸还原酶电极的研究[J]. J4, 2007, 37(2): 355-360.

ZHAO Lin, YU Yang, YAN Bo, TAN Xin, LI Ruo-pu. Amperometric Nitrate Reductase Biosensors Based on Organic-inorganic Hybrid Material[J]. J4, 2007, 37(2): 355-360.

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