Journal of Jilin University(Earth Science Edition) ›› 2021, Vol. 51 ›› Issue (4): 1217-1223.doi: 10.13278/j.cnki.jjuese.20200031

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Rapid Detection of Microcystin-LR Based on Green Biotemplated Electrode

Piao Yunxian, Yao Lan, He Lingzhi, Zhang Yu   

  1. College of New Energy and Environment, Jilin University/Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Changchun 130021, China
  • Received:2020-02-15 Online:2021-07-26 Published:2021-08-02
  • Supported by:
    Supported by the National Natural Science Foundation of China (51809111)

Abstract: For the effective detection of microcystin-LR (MCLR) in environmental water, in this study, the biochar particles were prepared from agricultural wastes,and it was utilized for the construction of a biotemplated electrode with electrochemical immunoassay capability toward microcystin-LR by differential pulse voltammetry. It is found that the electrode can identify the presence of microcystin-LR effectively in a short time (5 min), the linear detection range is 0.0-5.0 nmol/L and the lowest detection limit is 0.2 nmol/L. The electrode is also anti-interfere to various unrelated molecules and ions, indicating that the electrode can be used for fast and effective detection of microcystin-LR.

Key words: biochar, microcystin-LR, electrochemical detection

CLC Number: 

  • X502
[1] Han J H, Zhang J P, Xia Y T, et al. Highly Sensitive Detection of the Hepatotoxin Microcystin-LR by Surface Modification and Bio-nanotechnology[J]. Colloids and Surfaces:A:Physicochemical and Engineering Aspects, 2011, 391:184-189.
[2] 宋兴良,冯晓阳,李娜. 分散液液微萃取气相色谱-质谱法测定水体中痕量的微囊藻毒素[J]. 临沂大学学报, 2019, 41(6):1-8. Song Xingliang, Feng Xiaoyang, Li Na. Determination of Trace Microcystis Toxin in Water by Dispersive Liquid-Liquid Microextraction Gas Chromatography-Mass Spectrometry[J]. Journal of Linyi University, 2019, 41(6):1-8.
[3] Wang Y, Liu H, Liu X, et al. Histone Acetylation Plays an Important Role in MC-LR Induced Apoptosis and Cycle Disorder in Sd Rat Testicular Cells[J]. Chemosphere, 2020, 241:125073.
[4] 张俊,孟宪智,张世禄,等. 海河流域地表水中微囊藻毒素的测定[J]. 环境监测管理与技术, 2019, 31(5):40-42. Zhang Jun, Meng Xianzhi, Zhang Shilu, et al. Determination of Microcystin in Surface Water in Haihe River Basin[J]. Environmental Monitoring Management and Technology, 2019, 31(5):40-42.
[5] Zanato N, Talamini L, Silva T R, et al. Microcystin-LR Label-Free Immunosensor Based on Exfoliated Graphite Nanoplatelets and Silver Nanoparticles[J]. Talanta, 2017, 175:38-45.
[6] Zhao L F, Teng L, Zhang J, et al. Point-of-Care Detection of Microcystin-LR with a Personal Glucose Meter in Drinking Water Source[J]. Chinese Chemical Letters, 2019, 30(5):1035-1037.
[7] Covaci O I, Sassolas A, Alonso G A, et al. Highly Sensitive Detection and Discrimination of LR and YR Microcystins Based on Protein Phosphatases and an Artificial Neural Network[J]. Analytical and Bioanalytical Chemistry, 2012, 404(3):711-720.
[8] Loyprasert S, Thavarungkul P, Asawatreratanakul P, et al. Label-Free Capacitive Immunosensor for Microcystin-LR Using Self-Assembled Thiourea Monolayer Incorporated with Ag Nanoparticles on Gold Electrode[J]. Biosensors and Bioelectronics, 2008, 24(1):78-86.
[9] Zhang W, Han C S, Jia B P, et al. A 3D Graphene-Based Biosensor as an Early Microcystin-LR Screening Tool in Sources of Drinking Water Supply[J]. Electrochimica Acta, 2017, 236:319-327.
[10] 徐海滨,孙明,隋海霞, 等. 江西鄱阳湖微囊藻毒素污染及其在鱼体内的动态研究[J]. 卫生研究, 2003(3):192-194. Xu Haibin, Sun Ming, Sui Haixia, et al. Microcystin Contamination of Fish on Poyang Lake in Jiangxi Province[J]. Journal of Hygiene Research, 2003(3):192-194.
[11] 吴和岩, 郑力行, 苏瑾,等. 上海市供水系统微囊藻毒素LR含量调查[J]. 卫生研究, 2005(2):152-154. Wu Heyan, Zheng Lixing, Su Jin, et al. Survey on the Contamination of Microcystin-LR in Water Supply of Shanghai City[J]. Journal of Hygiene Research, 2005(2):152-154.
[12] 何岸檐, 杨伟, 周倩如,等. 重庆市城镇集中式供水水源水中微囊藻毒素污染健康风险评估[J]. 中国卫生检验杂志, 2019, 29(15):1881-1883. He Anyan, Yang Wei, Zhou Qianru, et al. Health Risk Assessment of Microcystis Pollution in Centralized Water Source in Chongqing[J]. Chinese Journal of Health Laboratory Technology, 2019, 29(15):1881-1883.
[13] Zhang L, Ping X, Yang Z. Determination of Microcystin-LR in Surface Water Using High-Performance Liquid Chromatography/Tandem Electrospray Ionization Mass Detector[J]. Talanta, 2004, 62(1):191-198.
[14] Wang S, Ge L, Song X, et al. Paper-Based Chemiluminescence ELISA:Lab-on-Paper Based on Chitosan Modified Paper Device and Wax-Screen-Printing[J]. Biosensors and Bioelectronics, 2012, 31(1):212-218.
[15] Zhang G P, Li C, Wu S Q, et al. Label-Free Aptamer-Based Detection of Microcystin-LR Using a Microcantilever Array Biosensor[J]. Sensors and Actuators:B:Chemical, 2018(260):42-47.
[16] Sassolas A, Catanante G, Fournier D, et al. Development of a Colorimetric Inhibition Assay for Microcystin-LR Detection:Comparison of the Sensitivity of Different Protein Phosphatases[J]. Talanta, 2011, 85(5):2498-2503.
[17] 朴云仙,祁小丽,王湘,等.基于核酸适配体功能化石墨纳米颗粒荧光探针的17β-雌二醇快速检测方法[J].吉林大学学报(地球科学版),2019, 49(4):1137-1144. Piao Yunxian, Qi Xiaoli, Wang Xiang, et al. Rapid Detection of 17β-Estradiol Based on Fluorescent Probe of Functionalized Graphite Nanoparticle with Aptamer[J]. Journal of Jilin University (Earth Science Edition),2019,49(4):1137-1144.
[18] Chen X, Zhang K, Zhou J, et al. Electrochemical Immunosensor Based on Colloidal Carbon Sphere Array[J]. Biosensors and Bioelectronics, 2010, 25(5):1130-1136.
[19] Dong X W, He L Z, Hu H, et al. Removal of 17β-Estradiol by Using Highly Adsorptive Magnetic Biochar Nanoparticles from Aqueous Solution[J]. Chemical Engineering Journal, 2018, 352:371-379.
[20] He L Z, Yang Y S, Kim J B, et al. Multi-Layered Enzyme Coating on Highly Conductive Magnetic Biochar Nanoparticles for Bisphenol a Sensing in Water[J]. Chemical Engineering Journal, 2020, 384:123276.
[21] Weller M G. Immunoassays and Biosensors for the Detection of Cyanobacterial Toxins in Water[J]. Sensors (Basel), 2013, 13(11):15085-15112.
[22] Wang L B, Ma W, Xu L G, et al. Nanoparticle-Based Environmental Sensors[J]. Materials Science and Engineering:R:Reports, 2010, 70(3/4/5/6):265-274.
[23] Wang L, Chen W, Xu D, et al. Simple, Rapid, Sensitive, and Versatile SWNT-Paper Sensor for Environmental Toxin Detection Competitive with ELISA[J]. Nano Lett, 2009, 9(12):4147-4152.
[24] Eissa S, Ng A, Siaj M, et al. Label-Free Voltammetric Aptasensor for the Sensitive Detection of Microcystin-LR Using Graphene-Modified Electrodes[J]. Analytical Chemistry, 2014, 86(15):7551-7557.
[25] Taghdisi S M, Danesh N M, Ramezani M, et al. A Novel Fluorescent Aptasensor for Ultrasensitive Detection of Microcystin-LR Based on Single-Walled Carbon Nanotubes and Dapoxyl[J]. Talanta, 2017, 166:187-192.
[26] Zhang Y L, Chen M, Li H Y, et al. A Molybdenum Disulfide/Gold Nanorod Composite-Based Electrochemical Immunosensor for Sensitive and Quantitative Detection of Microcystin-LR in Environmental Samples[J]. Sensors and Actuators:B:Chemical, 2017, 244:606-615.
[27] Gao Y, Pramanik A, Begum S, et al. Multifunctional Biochar for Highly Efficient Capture, Identification, and Removal of Toxic Metals and Superbugs from Water Samples[J]. ACS Omega, 2017, 2(11):7730-7738.
[28] Tan X, Liu Y, Zeng G, et al. Application of Biochar for the Removal of Pollutants from Aqueous Solutions[J]. Chemosphere, 2015, 125:70-85.
[29] Dong X W, He L Z, Liu Y, et al. Preparation of Highly Conductive Biochar Nanoparticles for Rapid and Sensitive Detection of 17Β-Estradiol in Water[J]. Electrochimica Acta, 2018, 292:55-62.
[30] Dong X W, He L Z, Hu H, et al. Removal of 17Β-Estradiol by Using Highly Adsorptive Magnetic Biochar Nanoparticles from Aqueous Solution[J]. Chemical Engineering Journal, 2018, 352:371-379.
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