关键词: 五味子水提物, 微波辐射, 肝脏, 大鼠, Wistar

Abstract:

Abstract:Objective To study the influence of schisandrae water extract in the oxidative stress injury of liver cells of the  rats induced by   microwave radiation, and to clarify the preventive effect of schisandrae water extract on hepatic injury induced by microwave radiation in  rats.Methods 36 normal adult Wistar rats were randomly divided into normal control group(n=12),microwave radiation group(n=12),and schisandrae water extract prevention group(n=12).The rats in schisandrae water extract prevention group were pretreated individually with schisandrae water extract for 5 d before  microwave radiation,after  microwave radiation and schisandrae water extract treatment,the concentration of MDA and the activity of  SOD of the rats 0 and 16 h after radation in various  groups were detected,and the morphological changes of the liver tissue and liver cells were  observed by HE staining.Results The concentration of MDA and the activity of SOD of the rats in microwave radiation group had no significant difference  compared with  normal control group 0 h after microwave radiation.16 h after   microwave radiation,the concentration of  MDA was increased(P<0.05),and the activity of SOD of the  rats in  microwave radiation group was obviously decreased(P<0.05).The concentration  of MDA was decreased and the activity of SOD was increased in schisandrae water extract prevention group (P<0.05). Microwave radiation casused obvious edema of the liver cells,but the pathological injury of the liver tissue  in the rats in schisandrae water extract prevention group was improved.Conclusion 200 mW?cm－2 microwave radiation can induce the changes of oxidative stress level and morphology of liver cells of the rats,and   schisandrae water extract shows obvious therapeutical effects on the  stress injury of liver cells in Wistar rats induced by microwave radiation.

Key words: schisandrae water extract, microwave radiation, liver, rat,Wistar