关键词: 活性成分, 镇痛, 巨噬细胞

Abstract: Abstract:Objective To prepare active constituent from Qianglitianmaduzhong capsule (QLTM) and observe the analgesic effect of active constituent on mice and regulatory effect on macrophages.Methods Mice were randomly divided into five groups: negative control group (normal saline group ),QLTM capsule group (positive control group),active constituent groups with high,middle,and low doses.The mice ware administered by oral gavage every day for 14 d.In hot-plate test,the beginning time and times of licking hind paws within 1 min of mice were observed. In acetic acid-induced writhing test,the beginning time and writhing times within 15 min of mice were observed. The phagocytic ability of peritoneal macrophage was evaluated with chicken red blood cell phagocytosis assay.Results In hot -plate test,the active constituent with high and middle doses prolonged the beginning time of licking hind paws and reduced times of licking hind paws within 1 min (P<0.05 or P<0.01),compared with negative control group.In acetic acid-induced writhing test,the active constituent with high and middle doses prolonged the beginning time of acetic acid-reduced writhing and reduced writhing times within 15 min (P<0.05).Both active constituent and QLTM inhibited the phagocytic ability of mouse peritoneal macrophages,especially in middle-dose group of active constituent,statistically significant difference was observed compared with negative control group (P<0.05).Conclusion The active constituent from QLTM has analgesic effect on mice and can inhibit activation of macrophages.

Key words: active constituent, analgesic effect, macrophages