关键词: 血小板减少, 疾病模型, 动物

Abstract: Abstract:Objective To study the treating effect of middle and high doses of acetone and aether extracts from Rumex.Japonicus Houtt on mouse thrombocytopenia.Methods 80 BACB/C mice were randomly divided into 5 groups　(n=16): negative control group(salad oil),middle dose (0.5 g•kg^-1) and high dose(5 g•kg^-1 )of acetone and aether extracts groups.All mice in various group were injected with cyclophos-phamide （0.3 mL/20 g） by hypodermic for 3 d to set up the thrombocytopenic model; after mouse thrombocytopenic model was successfully set up,the mice were given salad oil,middle and high doses of acetone and aether extracts,respectively, by gastric perfusion，20 d later the mice were executed,the numbers of the platelet,red cells and white cells were determined.Results ①The numbers of platelet numbers of all groups on the 14th day after making model decreased significantly(P<0.01)；②When the experiment was finished,the platelet numbers of mice were significantly increased compared with the 14th day(P< 0.01)，the platelet numbers of experimental groups were obviously increased compared with negative control group(P< 0.01)；③ There were no significant differences of the numbers of red cells and white cells between experimental groups and negative control group(P>0.05).Conclusion Middle and high doses of acetone and aether extracts have obviously curative effect on mouse thrombocytopenia.

Key words: thrombocytopenia, disease model, animal