关键词: 基因沉默, RNA干扰, 膀胱肿瘤

Abstract: Abstract:Objective To investigate the influence of HIWI gene silencing in biological behavior of T24 cells and explore the possibility for HIWI gene to be used as the molecular target of inhibiting bladder carcinoma cell proliferation with gene transfection and RNA interference (RNAi) technique.Methods T24 cells were divided into transfection group with pGenesil-2-HIWI,transfection group with pGenesil-2-HIWI2263,transfection group with pGensil-2-control,and two control groups transfected with PEI only, and PBS only, respectively.T24 cells were transfected with shRNA expression vectors targeting HIWI gene by PEI,and the cell proliferation and cell cycle were measured by MTT assay and FCM.Results At the time of post-transfection 24 h,the inhibitory rate of cell proliferation in transfection groups were 32.60% and 26.09%,they were lower than that in control group (3.54%). At the time of post-transfection 48 h,the percentages of cells at S phase in transfection groups were (29.39± 3.27)% and (30.87±10.88)%,they were lower than that in control group (39.36%±2.09%) (P<0.01),and the percentages of cells at G₂/M phase in transfection groups were (9.39±0.80)% and (11.46±1.53)%,they were higher than that in control group (5.57%±0.40%) (P<0.05).Conclusion HIWI gene silencing has inhibitory effect on the proliferation of T24 cells.HIWI gene could be a molecular target of inhibiting bladder carcinoma cell proliferation,and HIWI gene silencing has a protential treatment value in inhibiting bladder carcinoma cell proliferation.

Key words: gene silencing, RNA interference, bladder neoplasms