关键词: 牙周膜细胞; , Cp G ODN, 细胞增殖

Abstract:

Abstract:Objective To screen the optimal Cp G ODN for inducing the proliferation of human periodental ligament cells(PDLCs), and to provide the experimental basis for the application of Cp G ODN in treatment of periodentitis.Methods The 48 healthy premolars of young people(10-15 years old) undergoing tooth extraction for orthodontic treatment were collected.Tissue explant primary culture was used to cultivate the PDLCs in vitro.The cells from the 4th  to 5th passages were selected for experiment.1-12 Cp G ODN were respectively given to PDLCs for 24, 48, and 72 h , and the cells treated with PBS only were used as control group.MTT assay was used to detect the cell proliferation. The above experiments were repeated for 3 times.Results The human PDLCs of primary culture were obtained with tissue cultivation.The primary cultivated PDLCs showed long spindle appearance.By immunocytochemical analysis, these cells were positive to antibodies against vimentin,and negative to antibodies against ceratin, indicating themselves to be mesoderm-derived fibroblasts.The mean A values in Cp G ODN groups were all higher than those in PBS control group，and the A values of the  9th and 11th Cp G ODNs were significantly higher than that in PBS control group ( P<0.05 or P<0.01).Conclusion Cp G ODN can promote the proliferation of human PDLCs, and the 9th and 11th Cp G ODN can significantly promote the proliferation of human PDLCs.

Key words: periodental ligament cells; , Cp G ODN; , cell proliferation