关键词: 激光, 骨肉瘤, 抗药性, 多药, 增殖, 抑制

Abstract: Objective To explore the effects of semiconductor laser on proliferation of the multidrug-resistant model of human osteosarcoma cell line (R-OS-732). Methods Four test groups and one control group were set up. The test groups were divided into A,B,C and D groups according to the irradiation time (output power was 200 mW). The time of laser irradiation of A,B,C and D groups were 5,10, 20 and 40 min， repectively. The control group (E) wasn′t treated with laser irradiation. MTT method was used to observe the proliferation of R-OS-732， and the morphological changes of the cells were observed by inverted microscope. Results The survival rates of cells in all test groups under condition of semiconductor laser irradiation with 200 mW output power and 532 nm weavlength, compared with control group (P<0.05); and it increased with the irradiation dose of semiconductor laser. The cellular density decreased under light microscope. Conclusion The semiconductor laser can result in the inhibition of proliferation of R-OS-732 cultured in vitro.

Key words: lasers, osteosarcoma, drug resistance, multiple, inhibition, proliferation