关键词: 乳腺肿瘤, 神经生长因子, Neuritin, 实时荧光定量PCR法

Abstract:

Abstract:Objective To observe the expressions of neuritinrotrophic factor
 Neuritin protein and mRNA in human breast cancer tissue and the relationship between
 its expression and the clinicopathological features,and to explore its roles
 in the carcinogenesis and progression of breast cancer.Methods
74 cases of breast cancer tissue samples and 12 cases of normal breast tissue samples were coll
ected.The expressions of Neuritin protein in samples were detected with immunohistochemical assay and Western blotting method;the expression levels of Neuritin mRNA  in samples were detected by quantitative  RT-PCR.
Results The expression rate of Neuritin  in normal breast tissue was 25%，
and the positive expression rate of Neuritin in breast cancer tissue  was 70.62%,which was higher than that in norma
l breast tissue (P<0.05);Neuritin mainly located in the nucleus and cytoplasm.Meantime,there were positive correlations between the positive expression of Neuritin and lymph node metastasis,TNM stages and the positive rate of ER/PR/HER2.Compared with normal breast tissue,the expression levels of Neuritin protein and mRNA in stage Ⅰ-Ⅱ and Ⅲ-Ⅳ breast cancer  tissue were increased (P<0.05 or P<0.01);compared with stage Ⅰ-Ⅱ breast cancer tissue,the expresson leve
ls of Neuritin protein and mRNA in stage Ⅲ-Ⅳ breast cancer tissue was significantly increased(P<0.05).Conclusion Neuritin may be one
 of the genes to enhance the growth of breast cancer and may be a target for the treatment of breast cancer.



Key words: breast neoplasms, neuritinrotrophic factor, Neuritin, quantitative fluorescence real-time PCR