胎盘表达转录因子1在多囊卵巢综合征大鼠卵巢组织中的表达及其对大鼠卵巢颗粒细胞增殖的影响

doi:10.13481/j.1671-587X.20250503

Abstract

Abstract:

Objective To investigate the expression of placenta expressed transcript 1（Plet1）in ovarian tissue of the letrozole-induced model rats of polycystic ovary syndrome （PCOS） and its effect on the proliferation of rat ovarian granulosa cells，and to clarify the possible mechanism by which Plet1 may contribute to the pathogenesis of PCOS. Methods The ovarian tissue samples from the rats collected in previous studies were used and divided into control and PCOS groups. Real-time fluorescence quantitative PCR （RT-qPCR） and Western blotting methods were used to detect the expression levels of Plet1 mRNA and protein in ovarian tissue of the rat in two groups. Additionally， twenty-four rats underwent vaginal smear cytology were divided into four groups by estrous cycle phase： proestrus，estrus，metestrus and diestrus. RT-qPCR was used to detect the expression level of Plet1 mRNA in ovarian tissue of the rats in various groups，and immunohistochemistry （IHC） method was used to detect the location of Plet1 expression in the rat ovarian tissue in various groups. The rat ovarian granulosa cells were transfected and divided into control group， si-Plet1-rat-266 group， si-Plet1-rat-383 group， and si-Plet1-rat-554 group. Cell counting kit-8（CCK-8）method was used to assess the cell proliferation activities of rat ovarian granulosa cells in various groups， and RT-qPCR method was used to detect the expression levels of cyclin-dependent kinase 6 （CDK6） and P53 mRNA in rat ovarian granulosa cells in various groups. Results The RT-qPCR results revealed that Plet1 mRNA was expressed in the ovaries of normal rats， while no statistically significant difference was observed across estrous cycle phases （P>0.05）. The immunohistochemistry results showed that the expression of Plet1 protein was mainly localized in ovarian granulosa cells and luteal cells in the rat ovarian tissue. Compared with control group，the expression levels of Plet1 mRNA and protein in ovarian tissue of the rats in PCOS group were significantly decreased （P<0.05）. The RT-qPCR results showed that compared with control group， the expression level of Plet1 mRNA in ovarian granulosa cells in si-Plet1-rat-383 group was decreased （P<0.01）， exhibiting the most pronounced reduction. Compared with control group， the proliferation activity of rat ovarian granulosa cells in si-Plet1-rat-383 group was decreased （P<0.05）. Compared with control group， the expression levels of CDK6 and P53 mRNA in rat ovarian granulosa cells in si-Plet1-rat-383 group were significantly decreased （P<0.05 or P<0.01）. Conclusion Plet1 proteinis predominantly expressed and localized in granulosa cells and luteal cells in normal rat ovarian tissue. Its expression is downregulated in the ovarian tissue of PCOS model rats， and interference with Plet1 gene expression may inhibit the proliferation of rat ovarian granulosa cells.

Key words: Polycystic ovary syndrome, Placenta expressed transcription factor 1, Estrous cycle, Ovarian granulosa cells, Cell proliferation

CLC Number:

R711.7

Lulu FU,Yinggang ZOU,Xiaoyu ZHENG,Xueying ZHANG,Jingshun ZHANG,Min WANG,Qiang ZHANG,Lianwen ZHENG. Expression of placenta expressed transcription factor 1 in ovarian tissue of polycystic ovary syndrome rats and its effect on proliferation of rat ovarian granulosa cells[J].Journal of Jilin University(Medicine Edition), 2025, 51(5): 1177-1184.

Figures/Tables 9

Tab.1

Tab.2

Fig.?1

Fig.?2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Tab.3

References 23

[1]	ALENEZI S A， KHAN R， AMER S. The impact of high BMI on pregnancy outcomes and complications in women with PCOS undergoing IVF-a systematic review and meta-analysis［J］. J Clin Med， 2024， 13（6）： 1578.
[2]	COLLÉE J， MAWET M， TEBACHE L， et al. Polycystic ovarian syndrome and infertility： overview and insights of the putative treatments［J］. Gynecol Endocrinol， 2021， 37（10）： 869-874.
[3]	CHOUDHURY A A， RAJESWARI V D. Polycystic ovary syndrome （PCOS） increases the risk of subsequent gestational diabetes mellitus （GDM）： a novel therapeutic perspective［J］. Life Sci， 2022， 310： 121069.
[4]	曹欢易，关海霞，傅晓莹. 内分泌疾病与非酒精性脂肪性肝病［J］. 中国实用内科杂志， 2024， 44（1）： 11-16.
[5]	KARRICH J J， ROMERA-HERNÁNDEZ M， PAPAZIAN N， et al. Expression of Plet1 controls interstitial migration of murine small intestinal dendritic cells［J］. Eur J Immunol， 2019， 49（2）： 290-301.
[6]	MANSOURI-ATTIA N， AUBERT J， REINAUD P， et al. Gene expression profiles of bovine caruncular and intercaruncular endometrium at implantation［J］. Physiol Genomics， 2009， 39（1）： 14-27.
[7]	BROOKS K， BURNS G W， MORAES J G N， et al. Analysis of the uterine epithelial and conceptus transcriptome and luminal fluid proteome during the peri-implantation period of pregnancy in sheep［J］. Biol Reprod， 2016， 95（4）： 88.
[8]	ZHAO S H， SIMMONS D G， CROSS J C， et al. PLET1 （C11orf34）， a highly expressed and processed novel gene in pig and mouse placenta， is transcribed but poorly spliced in human［J］. Genomics， 2004， 84（1）： 114-125.
[9]	PERVIZAJ-ORUQAJ L， SELVAKUMAR B， FERRERO M R， et al. Alveolar macrophage-expressed Plet1 is a driver of lung epithelial repair after viral pneumonia［J］. Nat Commun， 2024， 15（1）： 87.
[10]	MURRAY A， SIENERTH A R， HEMBERGER M. Plet1 is an epigenetically regulated cell surface protein that provides essential cues to direct trophoblast stem cell differentiation［J］. Sci Rep， 2016， 6： 25112.
[11]	LUO M C， LAI W L， HE Z M， et al. Development of an optimized culture system for generating mouse alveolar macrophage-like cells［J］. J Immunol， 2021， 207（6）： 1683-1693.
[12]	EVERTS R E， CHAVATTE-PALMER P， RAZZAK A， et al. Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer［J］. Physiol Genomics， 2008， 33（1）： 65-77.
[13]	FU L L， XU Y， LI D D， et al. Expression profiles of mRNA and long noncoding RNA in the ovaries of letrozole-induced polycystic ovary syndrome rat model through deep sequencing［J］. Gene， 2018， 657： 19-29.
[14]	SIDDIQUI S， MATEEN S， AHMAD R， et al. A brief insight into the etiology， genetics， and immunology of polycystic ovarian syndrome （PCOS）［J］. J Assist Reprod Genet， 2022， 39（11）： 2439-2473.
[15]	PALOMBA S， PILTONEN T T， GIUDICE L C. Endometrial function in women with polycystic ovary syndrome： a comprehensive review［J］. Hum Reprod Update， 2021， 27（3）： 584-618.
[16]	STENER-VICTORIN E， TEEDE H， NORMAN R J， et al. Polycystic ovary syndrome［J］. Nat Rev Dis Primers， 2024， 10： 27.
[17]	TONG C， WU Y， ZHANG L L， et al. Insulin resistance， autophagy and apoptosis in patients with polycystic ovary syndrome： Association with PI3K signaling pathway［J］. Front Endocrinol， 2022， 13： 1091147.
[18]	AJMAL N， KHAN S Z， SHAIKH R. Polycystic ovary syndrome （PCOS） and genetic predisposition： a review article［J］. Eur J Obstet Gynecol Reprod Biol X， 2019， 3： 100060.
[19]	ZEPP J A， ZHAO J J， LIU C N， et al. IL-17A-induced PLET1 expression contributes to tissue repair and colon tumorigenesis［J］. J Immunol， 2017， 199（11）： 3849-3857.
[20]	KLOEPPER J E， BARIS O R， REUTER K， et al. Mitochondrial function in murine skin epithelium is crucial for hair follicle morphogenesis and epithelial-mesenchymal interactions［J］. J Invest Dermatol， 2015， 135（3）： 679-689.
[21]	ANDREWS N， SERVISS J T， GEYER N， et al. An unsupervised method for physical cell interaction profiling of complex tissues［J］. Nat Methods， 2021， 18（8）： 912-920.
[22]	LI J， WACHSMUTH L P， XIAO S Y， et al. Foxn1 overexpression promotes thymic epithelial progenitor cell proliferation and mTEC maintenance， but does not prevent thymic involution［J］. Development， 2023， 150（8）： dev200995.
[23]	TENG L， HONG L J， LIU R Z， et al. Cellular localization and regulation of expression of the PLET1 gene in porcine placenta［J］. Int J Mol Sci， 2016， 17（12）： 2048.

Primer	Sequence（5'-3'）
Plet1	F:AGCGGAGCATACGAGAAGTG
Plet1	R:TGTAGCTGTGCGGTTGATGA
GAPDH	F:CAAGTTCAACGGCACA
GAPDH	R:CAGTAGACTCCACGACAT

Primer	Sequence（5'-3'）
CDK6	F:GCACACATCAAACAACCTGACC
CDK6	R:GGCCCACTTCACCGTACTAA
P53	F:GCTGACCAGCAGTACGAATG
P53	R:GCACACATCAAACAACCTGACC
GAPDH	F:CAAGTTCAACGGCACA
GAPDH	R:CAGTAGACTCCACGACAT

Group	CDK6	P53
Control	1.037±0.080	1.050±0.014
Si-Plet1-rat-383	0.787±0.060^*	0.670±0.057^**

Expression of placenta expressed transcription factor 1 in ovarian tissue of polycystic ovary syndrome rats and its effect on proliferation of rat ovarian granulosa cells

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 9

References 23

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Xiao ZHOU,Haitao DAI,Yunshan DING,Linjie ZHANG,Nan WU. Network pharmacology analysis and in vitro experimental validation of anticancer effect of grape seed proanthocyanidins against oral squamous cell carcinoma [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 651-662.
[2]	Qiaotong REN,Weihua WU,Xingxiang WANG,Shichao WANG,Yipeng CHENG,Chun LI. Effect of interleukin-17 recepter B gene silencing on proliferation, invasion and apoptosis of lung adenocarcinoma A549 cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(3): 738-745.
[3]	Yao ZHENG,Mingxia FU,Weichen WANG,Weiwei CHEN,Yuchen HAN,Yu BAI,Jiajia AN. Effect of sodium selenite on biological behaviors of breast cancer doxorubicin-resistant MCF-7/ADR cells [J]. Journal of Jilin University(Medicine Edition), 2026, 52(2): 410-417.
[4]	Li ZHAI,Meng CHEN,Jianbo LUO,Aili ZHANG,Liangxiao WANG,Ying WEI,Xi ZHANG. Targeting relationship between miR-214-3p and EZH2 and its effects on proliferation， invasion， and apoptosis of ovarian cancer SKOV3 cells [J]. Journal of Jilin University(Medicine Edition), 2026, 52(2): 460-468.
[5]	Hong LI,Panpan YU,Zouyu ZHAO,Chongfeng SUN,Hui QIAO,Ping YANG. Expression of GSTO1 in ovarian cancer tissue and effect of N-glycosylation site mutations on biological behaviors of epithelial ovarian cancer cells [J]. Journal of Jilin University(Medicine Edition), 2026, 52(2): 469-482.
[6]	Yan ZHAO,Huawei WU. Inhibitory effect of inhibition of miR-17-5p on proliferation of spinal cord astrocytes of rats induced by scratch injury through targeting Mfn2 expression [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 81-92.
[7]	Xiaohan YAO,Mingchen YAO,Zhiqing WANG,Heyang LI,Yan YAN,Ningjing LEI. Effect of silencing GPR139 gene on proliferation, apoptosis and autophagy of breast cancer cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2026, 52(1): 1-9.
[8]	Sifan FENG,Yunfeng LI,Jiaying WANG,Fubin MA,Yan WANG. Effects of heme-binding protein 1 gene knockdown on proliferation, migration, and inflammatory response of microglia BV2 and their mechanisms [J]. Journal of Jilin University(Medicine Edition), 2025, 51(6): 1532-1541.
[9]	Ying HU,Yong HUANG. Improvement effect of short-chain fatty acids on inflammation-induced autophagic damage in ovarian granulosa cells in polycystic ovary syndrome and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(5): 1340-1348.
[10]	Yixuan GAO,Peng WANG,Silong ZHANG,Ruijuan GAO,Yingfang MA,Keke ZHANG,Dan FENG,Zongqi HUANG,Ketao MA,Li LI,Junqiang SI. Inhibitory effect of safranal on proliferation, migration and phenotypic transformation of vascular smooth muscle cells of rats induced by high glucose in vitro [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 948-957.
[11]	Xiaoshuang HE,Lina XU,Mei CUI,Yu ZHAO,Bei WANG,Zheng HUANG,Yuchao WANG,Wenyan XIN,Chao WU. Effects of lncRNA DUXAP8 in lung cancer A549 cells-derived exosomes on lung cancer cell growth and its mechnism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 958-967.
[12]	Hongli LI,Mengyao WANG,Yangyang LIU,Hui ZHANG,Li LI. Effect of KHSRP on biological behavior of colorectal cancer cells through activation of JAK/STAT signaling pathway [J]. Journal of Jilin University(Medicine Edition), 2025, 51(4): 996-1006.
[13]	Fan WANG,Xin WEN,Yixuan WANG,Yuan WANG. Effect of gap junction β2 on prognosis of patients with lung adenocarcinoma and biological behavior of lung adenocarcinoma A549 cells [J]. Journal of Jilin University(Medicine Edition), 2025, 51(3): 716-726.
[14]	Yihui WANG,Qing ZHANG,Yingnan LI,Liping YE. Effect of KIAA1522 on proliferation, migration, and invasion of lung cancer cells and its mechanism [J]. Journal of Jilin University(Medicine Edition), 2025, 51(3): 727-739.
[15]	Shuyan SUN,Huakun ZHANG,Ziru ZHOU,Feng LI,Xiaobin CUI. Expression of CRNN protein in esophageal squamous cell carcinoma tissue and influence of its overexpression in biological behavior of esophageal squamous cell carcinoma Eca9706 cells [J]. Journal of Jilin University(Medicine Edition), 2025, 51(2): 275-283.