Abstract:

Abstract:Objective
To study the expressions of nicotinamide phosphoribosyl transferase (Nampt) in main energy metabolism organs (liver,pancreas,skeletal muscle,and kidney) of the rats with type 2 diabetes mellitus (T2DM)， and to explore the correlation between the expression and distribution of Nampt and the occurrence of diabetes.Methods The SD rat diabetes model was established by injecting with streptozotcin(STZ).The SD rats were randomly divided into diabetes group and control group.Immunohistochemical Envision staining assay was used to detect the distribution and protein expressions of Nampt in liver,pancreas,muscle, and kidney tissues of the rats,at the same time the blood glucose and serum insulin levels were also be detected. Results The blood glucose level of the rats in diabetes  group was significantly higher than that in control group (P<0.01),and the fasting insulin level was lower than that in control group (P<0.01).The Nampt expression in the liver tissue of the rats in diabets group was significantly increased,which distributed near the hepatic sinus in diabetes rats,and the Nampt expression was also increased in skeletal muscle in which the whole cell  was thick dying;the Nampt expression  mainly distributed in the renal tubular epithelial cells.Compared with control group,the positive expression rates of Nampt  in liver,skeletal muscle, and kidney tissues of the rats in diabets group were significantly increased (P<0.05 or P<0.01). There was nearly no Nampt expression in pancreas tissue of the rats in diabetes group and the Nampt expression level was significantly lower than that in control  group (P<0.01).Conclusion The Nampt expressions are much different in main energy metabolic organs of the rats with diabetes.It issuggested that Nampt may be used as a specific indicative marker in the process of diabetes.